BACKGROUND Light is the main source of energy and, as such, is one of the most important environmental factors for plant growth, morphogenesis, and other physiological responses. MicroRNAs (miRNAs) are endogenous non-coding RNAs that contain 21-24 nucleotides (nt) and play important roles in plant growth and development as well as stress responses. However, the role of miRNAs in the light response is less studied. We used tomato seedlings that were cultured in red light then transferred to blue light for 2 min to identify miRNAs related to light response by high-throughput sequencing. RESULTS A total of 108 known miRNAs and 141 predicted novel miRNAs were identified in leaf samples from tomato leaves treated with the different light qualities. Among them, 15 known and 5 predicted novel miRNAs were differentially expressed after blue light treatment compared with the control (red light treatment). KEGG enrichment analysis showed that significantly enriched pathways included zeatin biosynthesis (ko00908), homologous recombination (ko03440), and plant hormone signal transduction (ko04075). Zeatin biosynthesis and plant hormone signal transduction are related to plant hormones, indicating that plant hormones play important roles in the light response. CONCLUSION Our results provide a theoretical basis for further understanding the role of miRNAs in the light response of plants.

中文翻译：

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不同光质量处理的番茄叶片中microRNA的差异表达。

背景技术光是主要的能量来源，因此，光是植物生长，形态发生和其他生理反应的最重要的环境因素之一。微小RNA（miRNA）是内源性非编码RNA，包含21-24个核苷酸（nt），在植物生长发育以及胁迫响应中发挥重要作用。但是，关于miRNA在光反应中的作用的研究较少。我们使用在红光下培养的番茄幼苗，然后转移到蓝光下2分钟，以通过高通量测序鉴定与光响应相关的miRNA。结果在用不同光质量处理过的番茄叶片中，共鉴定出108种已知的miRNA和141种预测的新miRNA。其中，与对照（红光处理）相比，蓝光处理后有15种已知和5种预测的新颖miRNA差异表达。KEGG富集分析显示，显着富集的途径包括玉米素合成（ko00908），同源重组（ko03440）和植物激素信号转导（ko04075）。玉米素的生物合成和植物激素信号转导与植物激素有关，表明植物激素在光响应中起重要作用。结论我们的结果为进一步了解miRNA在植物光反应中的作用提供了理论基础。和植物激素信号转导（ko04075）。玉米素的生物合成和植物激素信号转导与植物激素有关，表明植物激素在光响应中起重要作用。结论我们的结果为进一步理解miRNA在植物光反应中的作用提供了理论基础。和植物激素信号转导（ko04075）。玉米素的生物合成和植物激素信号转导与植物激素有关，表明植物激素在光响应中起重要作用。结论我们的结果为进一步了解miRNA在植物光反应中的作用提供了理论基础。