Gastric cancer is one of the common types of cancer around the world which has few therapeutic options. Nitrogen heterocyclic derivatives such as thiazoles are used as the basis for the progression of the drugs. The objective of this study was to synthesize the 1-((3-(4-chlorophenyl)-1-phenyl-1H-pyrazol-4-yl) methylene)-2-(4-phenylthiazol-2-yl) hydrazine (TP) conjugating with (3-Chloropropyl) trimethoxysilane (CPTMOS)-coated Fe3O4 nanoparticles (NPs) for anti-cancer activities against gastric AGS cancer cell line. The synthesized Fe3O4@CPTMOS/TP NPs were characterized by FT-IR, XRD, EDX, SEM, TEM and Zeta potential analyses. To evaluate the toxicity of the above compound after AGS cell culture in RPMI1640 medium, the cells were treated at different concentrations for 24 h. The viability of the cells was investigated by MTT assay. Moreover, apoptosis induced by Fe3O4@CPTMOS/TP NPs was assessed by Hoechst 33432 staining, oxygen activity specification evaluation, caspase-3 activity assay, cell cycle analysis and annexin V/PI staining followed by flow cytometry analysis. The IC50 value in AGS cells was estimated to be 95.65 µg/ml. The flow cytometry results of Fe3O4@CPTMOS/TP NPs revealed a large number of cells in the apoptotic regions compared to the control cells and the cells treated with TP. In addition, the amount of ROS production and caspase-3 activity increased in the treated cells with Fe3O4@CPTMOS/TP NPs. The percentage of inhibited cancer cells in the G0/G1 phase increased under the treatment in the binding state to the nonionic iron oxide nanoparticles. Overall, this study showed that Fe3O4@CPTMOS/TP NP had effect on induction of apoptosis and inhibiting the growth of AGS cancer cells. Thus, Fe3O4@CPTMOS/TP NP can be considered as a new anti-cancer candid for next phase of studies on mouse models.

中文翻译：

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通过3-氯丙基三甲氧基硅烷官能化的Fe3O4和1-（（3-（4-氯苯基）-1-苯基-1H-吡唑-4-基）亚甲基）-2-（4-苯基噻唑- 2-yl）肼：评估胃AGS癌细胞的抗癌性。

胃癌是世界上常见的癌症之一，几乎没有治疗选择。氮杂环衍生物如噻唑被用作药物发展的基础。这项研究的目的是合成1-（（3-（4-氯苯基）-1-苯基-1H-吡唑-4-基）亚甲基）-2-（4-苯基噻唑-2-基）肼（TP ）与（3-氯丙基）三甲氧基硅烷（CPTMOS）涂覆的Fe3O4纳米颗粒（NPs）结合，以对抗胃AGS癌细胞系的抗癌活性。通过FT-IR，XRD，EDX，SEM，TEM和Zeta电位分析对Fe3O4 @ CPTMOS / TP NPs进行了表征。为了评估在RPMI1640培养基中AGS细胞培养后上述化合物的毒性，将细胞以不同浓度处理24小时。通过MTT测定研究细胞的活力。此外，Fe3O4 @ CPTMOS / TP NPs诱导的细胞凋亡通过Hoechst 33432染色，氧活度规格评估，caspase-3活度测定，细胞周期分析和Annexin V / PI染色，然后进行流式细胞术分析进行评估。AGS细胞的IC50值估计为95.65 µg / ml。Fe3O4 @ CPTMOS / TP NPs的流式细胞术结果显示，与对照细胞和TP处理的细胞相比，凋亡区域中存在大量细胞。另外，用Fe 3 O 4 @ CPTMOS / TP NPs处理的细胞中ROS的产生量和caspase-3活性增加。在与非离子型氧化铁纳米粒子结合的状态下，在处理过程中，G0 / G1相中受抑制癌细胞的百分比增加。总体，这项研究表明，Fe3O4 @ CPTMOS / TP NP对诱导凋亡和抑制AGS癌细胞的生长具有作用。因此，Fe3O4 @ CPTMOS / TP NP可以被认为是小鼠模型下一阶段研究的新抗癌候选药物。