Multiple heavy metal-resistant bacterium, Micrococcus luteus strain AS2, was isolated from industrial waste water of District Sheikhupura, Pakistan. The isolated bacterium showed minimum inhibitory concentrations of 55 and 275 mM against arsenite and arsenate. The bacterial strain also showed resistance against other heavy metal ions, i.e., lead, cadmium, chromium, mercury, nickel, and zinc, apart from arsenic. The optimum temperature and pH were 37 °C and 7, respectively. The antioxidant enzymes such as catalase were significantly increased under arsenite stress. The increase in 43.9% of GSH/GSSG and 72.72% of non-protein thiol was determined under15 mM arsenite stress. Bacterial genome was sequenced through Illumina and Nanopore and genes related to arsenic and other heavy metals were identified and blast (tblastx) on NCBI. Through scanning electron microscopy, no morphological changes were observed in bacterial cells under arsenite stress. The peaks appeared in EDX showed that there is surface adsorption of arsenite in bacterial cell while it was confirmed from Fourier transformed infrared spectroscopy analysis that there is some interaction between arsenite and functional groups present on the surface of bacterial cell. The SDS-PAGE analysis of whole-cell proteins under 15 mM arsenite stress clearly revealed that there is upregulation of some proteins in ranged of 60 to 34 kDa. The bioremediation efficiency (E) of bacterial biomass was 72% after 2 h and 99% after 10 h. The bioremediation efficiency of bacterial biomass is an indicator for the isolated bacterium to employ as a potential candidate for the amelioration of sites contaminated with arsenic.

中文翻译：

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从工业废水中分离出的多种耐重金属的黄褐微球菌AS2的表型和基因组分析及其在砷生物修复中的潜在用途。

从巴基斯坦Sheikhupura区的工业废水中分离出多种耐重金属细菌，即黄曲霉微球菌AS2。分离出的细菌对亚砷酸盐和砷酸盐的最低抑制浓度为55和275 mM。除砷外，细菌菌株还显示出对其他重金属离子（如铅，镉，铬，汞，镍和锌）的抵抗力。最佳温度和pH分别为37°C和7。在亚砷酸盐胁迫下，抗氧化酶（如过氧化氢酶）显着增加。在15 mM亚砷酸盐胁迫下测定了GSH / GSSG和非蛋白硫醇的含量分别为43.9％和72.72％。通过Illumina和纳米孔对细菌基因组进行测序，并鉴定了与砷和其他重金属有关的基因，并在NCBI上进行了blast（tblastx）。通过扫描电子显微镜，在亚砷酸盐胁迫下在细菌细胞中未观察到形态变化。在EDX中出现的峰表明，细菌细胞中存在砷的表面吸附，而通过傅立叶变换红外光谱分析确认，细菌细胞表面上存在的砷与官能团之间存在一定的相互作用。在15 mM亚砷酸盐胁迫下对全细胞蛋白质的SDS-PAGE分析清楚地表明，某些蛋白质的上调范围为60至34 kDa。细菌生物量的生物修复效率（E）在2小时后为72％，在10小时后为99％。细菌生物质的生物修复效率是分离出的细菌用作缓解被砷污染的场所的潜在候选者的指标。