Komagataeibacter xylinus ( K. xylinus ) has been used for a long time as one of the main cellulose producers among bacterium. In order to gain a better understanding of the physiological and biochemical mechanisms of cellulose production, an efficient and non-invasive visualization method is highly demanded to monitor the morphological changes of K. xylinus during each stage of its development. In this study, a polarization parametric indirect microscopic imaging (PIMI) technique was applied to image the morphology variations of K. xylinus . The optical field with precisely controlled polarization status was incident on the sample and far-field images of the sample were taken under different illumination polarization. In the calculated parametric images, sub-diffraction features related to the morphology details of the K. xylinus were visualized with high contrast and spatial resolution, which is typically irresolvable in conventional microscopic...

中文翻译：

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偏光调制成像法表征木糖杆菌

木糖Komagataeibacter xylinus（K. xylinus）作为细菌中主要的纤维素生产商之一已使用了很长时间。为了更好地了解纤维素生产的生理和生化机理，迫切需要一种高效且无创的可视化方法来监测木糖K.xylinus在其发展的每个阶段的形态变化。在这项研究中，偏振参数间接显微镜成像（PIMI）技术被用于成像K. xylinus的形态变化。具有精确控制的偏振状态的光场入射到样品上，并且在不同的照明偏振下拍摄样品的远场图像。在计算出的参数图像中，子衍射特征与K的形态细节有关。