当前位置: X-MOL 学术Appl. Environ. Microb. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Genomic and phenotypic stability of Lactobacillus rhamnosus GG in an industrial production process
Applied and Environmental Microbiology ( IF 4.4 ) Pub Date : 2020-01-10
Stage, M., Gustafsson, A. W., Jorgensen, M., Vera-Jimenez, N. I., Wielje, M., Nielsen, D. S., Sandelin, A., Chen, Y., Baker, A.

Lactobacillus rhamnosus GG is one of the most widely marketed and studied probiotic strains. In L. rhamnosus GG the spaCBA-srtC1 gene cluster encodes pili, which are important for some of the probiotic properties of the strain. A previous study showed that the DNA sequence of the spaCBA-srtC1 gene cluster was not present in some L. rhamnosus GG variants isolated from liquid dairy products. To examine the stability of the L. rhamnosus GG genome in an industrial production process, we sequenced the genome of samples of L. rhamnosus GG (DSM 33156) collected at specific steps of the industrial production process, including the culture collection stock, intermediate fermentations, and final freeze-dried products. We found that the L. rhamnosus GG genome sequence was unchanged throughout the production process. Consequently, the spaCBA-srtC1 gene locus was intact and fully conserved in all 31 samples examined. In addition, different production batches of L. rhamnosus GG exhibited consistent phenotypes, including the presence of pili on final freeze-dried products and consistent characteristics on in vitro assays of probiotic properties. Our data show that L. rhamnosus GG is highly stable in this industrial production process.

Importance Lactobacillus rhamnosus GG is one of the best studied probiotic strains. One of the well-characterized features of the strain are the pili encoded by the spaCBA-srtC1 gene cluster. These pili are involved in persistence in the gastrointestinal tract and are important for the probiotic properties of L. rhamnosus GG. Previous studies demonstrated that the L. rhamnosus GG genome can be unstable under certain conditions and lose the spaCBA-srtC1 gene cluster. Since in vitro studies have shown that loss of the spaCBA-srtC1 gene cluster decreases certain L. rhamnosus GG probiotic properties, we assessed both the genomic stability and phenotypic properties of L. rhamnosus GG throughout an industrial production process. We found that neither genomic nor phenotypic changes occurred in the samples. Therefore, we demonstrate that L. rhamnosus GG retains the spaCBA-srtC1 cluster and exhibits excellent genomic and phenotypic stability in the specific industrial process examined here.



中文翻译:

鼠李糖乳杆菌GG在工业生产过程中的基因组和表型稳定性

鼠李糖乳杆菌GG是最广泛销售和研究的益生菌菌株之一。在鼠李糖乳杆菌GG中,spaCBA-srtC1基因簇编码菌毛,这对菌株的某些益生菌特性很重要。先前的研究表明,从液体乳制品中分离出来的某些鼠李糖乳杆菌GG变体中不存在spaCBA-srtC1基因簇的DNA序列。为了检查鼠李糖乳杆菌GG基因组在工业生产过程中的稳定性,我们对鼠李糖乳杆菌样品的基因组进行了测序GG(DSM 33156)是在工业生产过程的特定步骤中收集的,包括培养物收集储备,中间发酵和最终的冻干产品。我们发现鼠李糖乳杆菌GG基因组序列在整个生产过程中没有变化。因此,spaCBA-srtC1基因位点是完整的,并且在所有检查的31个样品中均完全保守。另外,鼠李糖乳杆菌GG的不同生产批次表现出一致的表型,包括最终冷冻干燥产物上存在菌毛以及在体外益生菌性质测定中具有一致的特征。我们的数据表明,鼠李糖乳杆菌GG在该工业生产过程中非常稳定。

重要性 鼠李糖乳杆菌GG是研究最多的益生菌菌株之一。该菌株的特征之一是由spaCBA-srtC1基因簇编码的菌毛。这些菌毛参与胃肠道的持久性,并且对于鼠李糖乳杆菌GG的益生菌性质很重要。先前的研究表明,鼠李糖乳杆菌GG基因组在某些条件下可能不稳定,并失去了spaCBA-srtC1基因簇。由于体外研究表明,spaCBA-srtC1基因簇的缺失会降低某些鼠李糖乳杆菌GG益生菌特性,我们在整个工业生产过程中评估了鼠李糖乳杆菌GG的基因组稳定性和表型特性。我们发现样品中既没有基因组变化也没有表型变化。因此,我们证明鼠李糖乳杆菌GG保留了spaCBA-srtC1簇,并在此处讨论的特定工业过程中表现出出色的基因组和表型稳定性。

更新日期:2020-01-13
down
wechat
bug