Platelets play crucial roles in hemostasis and immunity. Over the last decades, clinical evidence has revealed the significance of platelets as complementary biomarkers for the detection and treatment of various diseases, including cancer. Due to a lack of well standardized convenient isolation methods for platelets, pre-analytical factors such as complex handling procedures negatively impact the quality of the platelet samples, including overactivation, low purity, and poor reproducibility. This may lead to biased interpretation of various downstream analyses, such as proteomic and genomic analyses. Herein, we describe a fully automated lab-on-a-disc-based method of platelet isolation from a small volume of blood (<1 mL). This method provides higher yields (>4 folds) and purity (>99%) and lower platelet activation than the conventional method. Moreover, it was also superior in the detection of platelet-related RNAs CD41, PF4, and P2Y12 due to lower contamination with white blood cells.

中文翻译：

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离心微流控设备上的全自动血小板分离技术，用于分子诊断。

血小板在止血和免疫中起关键作用。在过去的几十年中，临床证据表明血小板作为检测和治疗包括癌症在内的各种疾病的补充生物标志物的重要性。由于缺乏用于血小板的标准化便捷分离方法，因此分析前的因素（例如复杂的处理程序）会对血小板样品的质量产生负面影响，包括活化过度，纯度低和重现性差。这可能导致对各种下游分析（如蛋白质组学和基因组分析）的解释有偏差。在本文中，我们描述了一种基于自动化的基于光盘的从少量血液（<1 mL）中分离血小板的方法。此方法提供更高的产量（> 4倍）和纯度（> 99％），并且血小板活化率低于传统方法。此外，由于白细胞污染较少，因此在血小板相关RNA CD41，PF4和P2Y12的检测中也很出色。