Dietary methionine restriction (MR) could prevent age-related diseases and extend lifespan. The present study compared the effects of different levels of MR on oxidative stress and behavioral deficits in middle-aged mice fed a diet with various contents of fat. C57BL/6J middle-aged male mice were divided into nine groups with various combinations of dietary fat (low, medium, and high) and MR (0%, 60%, and 80%). MR attenuated dietary fat-induced oxidative stress and anxiety; improved athletic ability, learning, memory, and cognitive ability in conjunction with an improved thyroid function in a dose-dependent manner. Moreover, the beneficial effect of MR60 was prominent in middle-aged mice fed a HF diet relative to the corresponding MR0 group. These results provide more information on the appropriate methionine content to design personalized nutritional diets.

饮食中的蛋氨酸限制（MR）可以预防与年龄有关的疾病并延长寿命。本研究比较了不同水平的MR对以不同脂肪含量饮食喂养的中年小鼠的氧化应激和行为缺陷的影响。C57BL / 6J中年雄性小鼠分为9组，分别具有饮食脂肪（低，中和高）和MR（0％，60％和80％）的各种组合。MR减轻了饮食中脂肪引起的氧化应激和焦虑；改善的运动能力，学习，记忆和认知能力，并以剂量​​依赖的方式改善甲状腺功能。此外，相对于相应的MR0组，在接受HF饮食的中年小鼠中MR60的有益作用非常明显。