Salmonella spp., one of the major causes of foodborne diseases and a public health burden worldwide. Lytic bacteriophages NINP13076 and NINP1162 infecting Salmonella spp were isolated from sewage water with host strains Salmonella enteritidis (ATCC 13076) and Salmonella spp (MTCC 1162) respectively. The morphology of phages by Transmission electron microscopy revealed that they belong to the family Siphoviridae. Turbidometric assay, spot test assay, and plating methods done to see the effect of bacteriophages on Salmonella in-vitro. The impact of bacteriophage NINP13076 on Salmonella in vitro at different multiplicity of infection by plating method indicated that significant reduction (8.3–5.3 log CFU/g) in Salmonella count observed at 10¹ multiplicity of infection. Raw chicken skin contaminated with Salmonella spp, treated with bacteriophages showed significant reduction (6.7–5.4 log CFU/g) of Salmonella after 3h of incubation at room temperature when compared to untreated control. The results of the experiment on the lytic effect of NINP13076 phages on Salmonella enteritidis (log CFU/ml) in experimentally contaminated carrot salads showed a significant reduction in Salmonella population (1 log) after 4h of incubation. These results show the potential effectiveness of this bacteriophage as a biocontrol agent against Salmonella spp in raw chicken skin and carrot salad.

沙门氏菌属，是食源性疾病的主要原因之一，也是全世界公共卫生负担的原因。从宿主菌株肠炎沙门氏菌（ATCC 13076）和沙门氏菌（MTCC 1162）分别从污水中分离出感染沙门氏菌的溶菌噬菌体NINP13076和NINP1162 。通过透射电子显微镜观察噬菌体的形态，表明它们属于Siphoviridae家族。进行浊度分析，斑点测试和铺板方法以观察噬菌体对沙门氏菌的体外影响。噬菌体NINP13076对沙门氏菌的体外影响接种方法在不同感染复数下的感染表明沙门氏菌计数在10 ¹感染复数下显着降低（8.3–5.3 log CFU / g）。沾染生鸡皮沙门菌属，用噬菌体治疗显示出显著减少（6.7-5.4日志CFU / g）的沙门氏菌与未处理的对照，当在室温下温育3小时后。NINP13076噬菌体对受实验污染的胡萝卜沙拉中肠炎沙门氏菌（log CFU / ml）的裂解作用的实验结果表明沙门氏菌明显减少孵育4小时后的种群（1 log）。这些结果表明，该噬菌体作为生鸡肉皮肤和胡萝卜沙拉中沙门氏菌的生防剂的潜在功效。