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Aligned Biomimetic Scaffolds based on Carbon Nanotubes-reinforced polymeric nanofibers for knee meniscus tissue engineering
Materials Letters ( IF 3 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.matlet.2020.127351
Thiago D. Stocco , Eliane Antonioli , Maria L. Romagnolli , Gustavo F. Sousa , Mario Ferretti , Anderson O. Lobo

Abstract Many strategies in tissue engineering have been developed in order to provide potential treatments for Knee meniscal injuries since it is a high incidence lesion and without effective treatment available in clinical practice. The success of this approach is directly related to the creation of a scaffold able to reproduce the complex extracellular matrix of the native meniscus with adequate mechanical properties. Here we fabricated an aligned biomimetic scaffold, based on carbon nanotube (CNT)-reinforced polymeric nanofibers. For that, scaffolds with both circumferentially and radially aligned polycaprolactone fibers were developed from a specific electrospinning setup and incorporated with two different concentrations of CNT: 0.05% and 0.10%. Characterization by scanning electron microscopy confirmed the spatial distribution and the alignment of the electrospun nanofibers as well as a decrease in average fiber size with the addition of CNT (the presence of CNT was confirmed by transmission electron microscopy). Dynamic mechanical analysis and biological assays showed an improvement in the mechanical properties related to increased CNT content without influence in mesenchymal stem cells survival. These findings suggest a potential applicability of this nanocomposite for knee meniscus tissue engineering.

中文翻译:

基于碳纳米管增强聚合物纳米纤维的对齐仿生支架用于膝关节半月板组织工程

摘要 组织工程中的许多策略已经开发出来,以便为膝关节半月板损伤提供潜在的治疗方法,因为它是一种高发病率的病变,并且在临床实践中没有有效的治疗方法。这种方法的成功与创建能够以足够的机械性能重现天然半月板的复杂细胞外基质的支架直接相关。在这里,我们制造了一个基于碳纳米管 (CNT) 增强聚合物纳米纤维的对齐仿生支架。为此,具有周向和径向排列的聚己内酯纤维的支架是从特定的静电纺丝装置开发的,并结合了两种不同浓度的 CNT:0.05% 和 0.10%。扫描电子显微镜的表征证实了电纺纳米纤维的空间分布和排列,以及添加 CNT 后平均纤维尺寸的减小(透射电子显微镜证实了 CNT 的存在)。动态力学分析和生物测定表明,与增加的 CNT 含量相关的力学性能有所改善,而不影响间充质干细胞的存活。这些发现表明这种纳米复合材料在膝关节半月板组织工程中具有潜在的适用性。动态力学分析和生物测定表明,与增加的 CNT 含量相关的力学性能有所改善,而不影响间充质干细胞的存活。这些发现表明这种纳米复合材料在膝关节半月板组织工程中具有潜在的适用性。动态力学分析和生物测定表明,与增加的 CNT 含量相关的力学性能有所改善,而不影响间充质干细胞的存活。这些发现表明这种纳米复合材料在膝关节半月板组织工程中具有潜在的适用性。
更新日期:2020-04-01
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