Abstract For tissue fabrication and tissue regeneration, three-dimension (3D) hydrogel matrix with both cellular bioactivity and versatile fabrication ability for in-situ cell laden is always challenging and spotlight. In this study, we establish a facile approach to modify gellan gum (GG) for overcoming its disadvantages, including high gelling temperature which is incompatible for in-situ cell encapsulation and the native inactivity for cell adhesion. By esterification reaction, methacrylic anhydride modified GG (MAGG) is prepared, producing photo-reactivity for crosslinking and grafting with RGD-contained peptides. Therefore, RGD-grafted hydrogel (MAGG-RGD) is feasibly prepared. Detail characterizations show that MAGG hydrogel possesses improved physicochemical properties, with tunable strength varied with MA dose. Moreover, the introduction of RGD domains significantly improves the MG63 cells proliferation, adhesion and spreading both on two-dimension (2D) surface and in 3D matrix. Given the native photo-crosslinkable capacity, tunable physicochemical nature and good bioactivity, MAGG-RGD hydrogel is perspective in tissue fabrication and tissue engineering, and expands the potential material platform for cell carrier or tissue scaffold.

中文翻译：

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甲基丙烯酸甲酯和含RGD多肽的双功能改性结冷胶水凝胶

摘要 对于组织制造和组织再生，具有细胞生物活性和多功能原位细胞制造能力的三维 (3D) 水凝胶基质一直是具有挑战性和聚光灯的领域。在这项研究中，我们建立了一种简便的方法来修饰结冷胶 (GG) 以克服其缺点，包括与原位细胞封装不相容的高胶凝温度和细胞粘附的天然惰性。通过酯化反应，制备了甲基丙烯酸酐改性的 GG（MAGG），产生光反应性，用于与含 RGD 的肽交联和接枝。因此，制备了 RGD 接枝水凝胶（MAGG-RGD）是可行的。细节表征表明，MAGG 水凝胶具有改进的理化性质，可调节强度随 MA 剂量而变化。而且，RGD 域的引入显着改善了 MG63 细胞在二维 (2D) 表面和 3D 基质中的增殖、粘附和扩散。鉴于天然的光交联能力、可调的物理化学性质和良好的生物活性，MAGG-RGD 水凝胶在组织制造和组织工程方面具有前景，并扩展了细胞载体或组织支架的潜在材料平台。