The present study on Bacillus coagulans strain LBSC (DSM 17654) describes the use of whole genome sequencing, in correlation with the phenotypic properties to assess the safety of the strain. Analysis of the 16S rRNA sequence of the B. coagulans strain LBSC (DSM 17654), showed 100% homology with 99% coverage with B. coagulans strain HM-08. BLAT (BLAST Like Analysis Tool) analysis for whole genome comparison with B. coagulans ATCC 7050, B. coagulans HM-08 and B. coagulans Slac showed 96%, 99% and 99% sequence identity respectively. Whole genome sequencing results demonstrated a single scaffold of 36,35,902 bp and 3331 coding sequences. Gene ontology segregated the proteins as those with molecular function, cellular component and biological process of the predicted genes from assembled genome. Risk associated sequences like antibiotic resistance genes, biogenic amine producing genes, virulence factor genes and other safety related genes were identified with focus on horizontal gene transfer and its non-functionality. The absence of mobile elements in the vicinity of the genes, render it non-transferable and non-toxic phenotypic properties confirm the non-functionality of the genes. Absence of functional genes of concern and confirmation of absence of mobile elements in the vicinity of other non-clinically significant genes indicated no safety concern. The absence of complete and functional prophage sequences which are deleterious for the genome stability and presence of CRISPR system which are advantageous for genome stability by acting as a barrier to entry of foreign DNA elements indicated the stability of the genome. The molecular approach used in this study satisfies the requirements for the safety assessment of the probiotic strain which could indicate it to be potentially safe.

在本研究中凝结芽孢杆菌菌株LBSC（DSM 17654）描述了使用全基因组测序的，在相关性与表型特性来评估所述菌株的安全性。对凝结芽孢杆菌菌株LBSC（DSM 17654）的16S rRNA序列的分析显示，凝结芽孢杆菌菌株HM-08具有100％的同源性和99％的覆盖率。BLAT（BLAST样分析工具）分析，用于与凝结芽孢杆菌ATCC 7050，凝结芽孢杆菌HM-08和凝结芽孢杆菌进行全基因组比较Slac分别显示出96％，99％和99％的序列同一性。全基因组测序结果显示了一个36,35,902 bp的单一支架和3331个编码序列。基因本体将蛋白质与具有蛋白质功能的蛋白质分离开来，这些蛋白质具有分子功能，细胞成分以及来自组装基因组的预测基因的生物学过程。确定了风险相关序列，如抗生素抗性基因，生物胺生成基因，毒力因子基因和其他与安全相关的基因，重点是水平基因转移及其非功能性。基因附近没有可移动的元素，使其具有不可转移性和无毒的表型特性，从而证实了基因的非功能性。所关注的功能基因的缺乏和其他非临床上重要基因附近的移动元件的存在的确认表明，没有安全隐患。缺乏对基因组稳定性有害的完整的和功能性的噬菌体序列，以及通过充当外来DNA元件进入的屏障而有利于基因组稳定性的CRISPR系统的存在表明了基因组的稳定性。这项研究中使用的分子方法满足了对益生菌菌株安全性评估的要求，这可能表明它是潜在安全的。缺乏对基因组稳定性有害的完整的和功能性的噬菌体序列，以及通过充当外来DNA元件进入的屏障而有利于基因组稳定性的CRISPR系统的存在表明了基因组的稳定性。这项研究中使用的分子方法满足了对益生菌菌株安全性评估的要求，这可能表明它是潜在安全的。缺乏对基因组稳定性有害的完整的和功能性的噬菌体序列，以及通过充当外来DNA元件进入的屏障而有利于基因组稳定性的CRISPR系统的存在表明了基因组的稳定性。这项研究中使用的分子方法满足了对益生菌菌株安全性评估的要求，这可能表明它是潜在安全的。