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An activatable liposomal fluorescence probe based on fluorescence resonance energy transfer and aggregation induced emission effect for sensitive tumor imaging.
Colloids and Surfaces B: Biointerfaces ( IF 5.8 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.colsurfb.2020.110789
Yuqiong Xia 1 , Chunzhong Xu 1 , Xianghan Zhang 1 , Jingkai Gao 1 , Yankun Wu 1 , Cairu Li 1 , Zhongliang Wang 1
Affiliation  

Liposomes are of great interest and importance in tumor imaging, since they can greatly improve the imaging sensitivity and specificity by increasing the accumulation of contrast agents. Still, most liposome-based probes have high background signals during blood circulation, which limits enhancement of S/B ratio and tumor imaging sensitivity. To enhance the S/B ratio of tumor imaging, we construct a fluorescence resonance energy transfer (FRET) and aggregation induced emission (AIE) based liposomal fluorescence probe TPE/BHQ-lipo with excellent FRET effect (99 %) and great fluorescence enhancement upon liposome rupture (120-fold) as well as efficient fluorescence recovery in tumor cell imaging. Finally, we used the TPE/BHQ-lipo to image 4T1 tumor upon intravenous injection of liposomes and the group showed enhanced signal to background ratio of 4.1, compared to 1.8 from control AIE-based liposomal group (TPE-lipo). Our work offers an excellent FRET and AIE-based liposomal probe for high-sensitive tumor imaging.

中文翻译:

基于荧光共振能量转移和聚集诱导的发射效应的可活化脂质体荧光探针,用于敏感的肿瘤成像。

脂质体在肿瘤成像中具有极大的意义和重要性,因为它们可以通过增加造影剂的积累来大大提高成像灵敏度和特异性。尽管如此,大多数基于脂质体的探针在血液循环中仍具有很高的背景信号,这限制了S / B比和肿瘤成像灵敏度的提高。为了提高肿瘤成像的S / B比,我们构建了基于荧光共振能量转移(FRET)和聚集诱导发射(AIE)的脂质体荧光探针TPE / BHQ-lipo,具有出色的FRET效果(99%),并具有很强的荧光增强作用。脂质体破裂(120倍)以及肿瘤细胞成像中有效的荧光恢复。最后,我们使用TPE / BHQ-lipo对静脉注射脂质体的4T1肿瘤成像,该组显示出增强的信噪比4。如图1所示,与基于对照AIE的脂质体组(TPE-lipo)的1.8相比。我们的工作提供了出色的FRET和基于AIE的脂质体探针,用于高灵敏度肿瘤成像。
更新日期:2020-01-13
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