Thiophenol has a broad application in agriculture and industry. However, thiophenol can harm to the environment and health for its high toxicity. Developing an effective method for detection of thiophenol in the field of environmental and biology is valuable. In this work, we construct a reaction-based ratiometric fluorescent probe (E)-4-(2-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)vinyl)-1-(4-(2,4-dinitrophenoxy)benzyl)pyridin-1-ium bromide (DCVP-DNP) for probing thiophenol in environment and cells by employing (E)-7-(diethylamino)-3-(2-(pyridin-4-yl)vinyl)-2H-chromen-2-one (DCVP) as the fluorophore and 2,4-dinitrophenyl (DNP) ether as the recognition group for the first time. The probe has high selectivity for thiophenol though thiophenol-triggered nucleophilic substitution reaction. In addition, the ratio of emission intensities of the probe has linearly with thiophenol concentration in the range of 0-65 μM and the detection limit of thiophenol is as low as 4.8 × 10-8 M. Moreover, the probe can not only be applied for detection of thiophenol in water samples, but also image thiophenol in living cells, suggesting its potential application in environment and biological system.

中文翻译：

---

一种用于硫酚可视化的比例荧光探针及其应用。

硫酚在农业和工业中具有广泛的应用。但是，苯硫酚的高毒性会危害环境和健康。开发一种在环境和生物学领域中检测苯硫酚的有效方法非常有价值。在这项工作中，我们构建了一个基于反应的比例荧光探针（E）-4-（2-（7-（二乙氨基）-2-oxo-2H-chromen-3-yl）乙烯基）-1-（4-（ 2,4-二硝基苯氧基）苄基）吡啶-1-溴化铵（DCVP-DNP）通过使用（E）-7-（diethylamino）-3-（2-（pyridin-4-yl））探测环境和细胞中的苯酚乙烯基）-2H-铬-2--2-酮（DCVP）作为荧光基团和2,4-二硝基苯基（DNP）醚作为识别基团。通过硫酚引发的亲核取代反应，该探针对硫酚具有高选择性。此外，