Abstract Platelets can sense their surroundings as they crawl in search of optimal surface to adhere before activation, but the detailed mechanism has not been fully explored in different microenvironments. Herein, various aspects of platelet behavior, including morphology, movement and biomarker expression, were multilaterally examined on mechanically stimulating surfaces, with controlled rigidity and roughness, to elucidate the effect of microenvironment without chemical stimulus. The platelets demonstrated different movement behavior (e.g. back-and-forth and forward crawling), and motility, as well as varying degrees of spreading with lamellipodial and filopodial projections, based on varying mechanical microenvironments. Furthermore, after initial activation, the platelets all became stationary and the cell spreading became more prominent, while still maintaining their movement potential. P-selectin expression, along with α-granule and open canalicular system (OCS) distributions, all supported the morphological and movement behavior. These results strongly indicated that the mechanical microenvironment played a key role in the regulation of complex platelet activities.

中文翻译：

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用于复杂血小板行为的片上分析的机械刺激微平台的精细制造

摘要 血小板在激活前爬行以寻找最佳粘附表面时可以感知周围环境，但其在不同微环境中的详细机制尚未得到充分探索。在这里，血小板行为的各个方面，包括形态、运动和生物标志物表达，在机械刺激表面上进行了多方面的检查，具有受控的刚度和粗糙度，以阐明没有化学刺激的微环境的影响。基于不同的机械微环境，血小板表现出不同的运动行为（例如来回和向前爬行）和运动性，以及不同程度的片状和丝状突起的扩散。此外，在初始激活后，血小板都变得静止，细胞扩散变得更加突出，同时仍保持其运动潜力。P-选择素表达，以及α-颗粒和开放小管系统（OCS）分布，都支持形态学和运动行为。这些结果有力地表明，机械微环境在复杂血小板活性的调节中起关键作用。