Perfluorinated organic compounds (PFCs) are nontoxic, biocompatible, bioavailable, and bioorthogonal species which possess the unique ability to segregate away from both polar and nonpolar solvents producing a compact fluorophilic phase. Traditional techniques of fluorous chemical proteomics are generally applied to enrich biological samples in target protein(s) exploiting this property of PFCs to build fluorinated probes able to covalently bind to protein ensembles and being selectively extracted by fluorophilic solvents. Aiming at building a strategy able to avoid irreversible modification of the analyzed biosystem, a novel fully noncovalent probe is presented as an enabling tool for the recognition and isolation of biological protein(s). In our strategy, both the fluorophilic extraction and the biorecognition of a selected protein successfully occur via the establishment of reversible but selective interactions.

中文翻译：

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用于非共价蛋白识别和分离的全氟化探针。

全氟有机化合物（PFC）是无毒的，生物相容性的，可生物利用的和生物正交的物种，具有独特的分离极性和非极性溶剂的能力，从而形成致密的氟相。通常采用传统的含氟化学蛋白质组学技术，利用PFC的这一特性来富集靶蛋白中的生物样品，以构建能够共价结合蛋白质组的含氟探针，并由含氟溶剂选择性提取。为了建立一种能够避免对所分析生物系统进行不可逆修改的策略，提出了一种新型的完全非共价探针，作为一种识别和分离生物蛋白的实现工具。在我们的策略中