Molecularly imprinted film coated gold nanoprticles (MIP-AuNPs) were employed as surface-enhanced Raman scattering (SERS) substrate for sensitive and selective recognition and quantification of l-Phenylalanine (L-Phe). The MIP was in-situ formed on the AuNPs by sol-gel technique using L-Phe as the template molecule, tetraethyl orthosilicate as the crosslinker and phenyltrimethoxysilane as the functional monomer. The efficient removal of template was achieved by ultrasonic treatment. The as-prepared MIP-AuNPs sensor showed a good linear relationship with the concentration of L-Phe in the range of 1.0 × 10-8-1.0 × 10-4 mol L-1 with a limit of detection as low as 1.0 nmol L-1. The sensor also showed an excellent selectivity as L-Phe can be determined in the presence of other amino acid analogues, D-Phe and bovine serum. The stability of the MIP-AuNPs was manifested by the low deviation (RSD = 3.7%) for 40 days subsequent measurements.

中文翻译：

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通过表面增强的拉曼散射，通过分子印迹聚合物涂覆的Au纳米颗粒对l-苯丙氨酸进行高选择性检测。

分子印迹膜包被的金纳米颗粒（MIP-AuNPs）被用作表面增强拉曼散射（SERS）底物，用于灵敏和选择性地识别和定量I-苯丙氨酸（L-Phe）。通过使用L-Phe作为模板分子，原硅酸四乙酯作为交联剂和苯基三甲氧基硅烷作为功能单体，通过溶胶-凝胶技术在AuNPs上原位形成MIP。通过超声处理有效地去除了模板。所制备的MIP-AuNPs传感器与L-Phe浓度在1.0×10-8-1.0×10-4 mol L-1范围内表现出良好的线性关系，检出限低至1.0 nmol L -1。该传感器还显示出极好的选择性，因为可以在其他氨基酸类似物，D-Phe和牛血清的存在下测定L-Phe。