To evaluate the effect of drug hydrophobicity on nanoparticle delivery in vivo, we conducted a comparative study using different photosensitizer-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs). Chlorin e6 (Ce6) and pheophorbide a (Pba) with similar structure but different hydrophobicity were loaded into PLGA-NPs separately. We observed release profiles and photodynamic effects in vitro from the resulting Ce6- and Pba-PLGA-NPs. After intravenous injection into SCC7 tumor-bearing mice, biodistribution and accumulation of two drugs in tumor tissue were observed by real-time fluorescence imaging. Finally, in vivo photodynamic therapy with Ce6- and Pba-PLGA-NPs provided different therapeutic results according to imaging data. The results demonstrated that drug hydrophobicity is an important factor in nanoparticle drug delivery and should be considered for efficient drug delivery in vivo.

中文翻译：

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使用两种光敏剂对药物疏水性对体内纳米颗粒药物递送的影响进行比较研究。

为了评估药物疏水性对体内纳米颗粒递送的影响，我们进行了一项比较研究，使用不同的光敏剂负载的聚乳酸-乙醇酸共聚物（PLGA）纳米颗粒（NPs）。将结构相似但疏水性不同的氯绿素e6（Ce6）和脱镁叶绿素a（Pba）分别加载到PLGA-NP中。我们观察到从产生的Ce6-和Pba-PLGA-NPs的释放曲线和体外光动力效应。静脉注射到SCC7荷瘤小鼠后，通过实时荧光成像观察两种药物在肿瘤组织中的生物分布和积累。最后，根据成像数据，使用Ce6-和Pba-PLGA-NP进行的体内光动力疗法可提供不同的治疗效果。