Enhanced neuronal differentiation of neural stem cells with mechanically enhanced touch-spun nanofibrous scaffolds.,Nanomedicine: Nanotechnology, Biology and Medicine

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Enhanced neuronal differentiation of neural stem cells with mechanically enhanced touch-spun nanofibrous scaffolds.
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 5.4 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.nano.2020.102152
Darya Asheghali ₁ , Se-Jun Lee ₂ , Andreas Furchner ₃ , Alexey Gruzd ₄ , Steven Larson ₅ , Alexander Tokarev ₆ , Seth Stake ₇ , Xuan Zhou ₂ , Karsten Hinrichs ₃ , Lijie G Zhang ₈ , Sergiy Minko ₉

Affiliation

Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA; Department of Chemistry, The University of Georgia, Athens, GA, USA.
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA.
Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Berlin, Germany.
Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA; Graduate Program of Polymer, Fibers, and Textile Sciences, USA.
Department of Physics and Astronomy, The University of Georgia, Athens, GA, USA.
Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA.
Department of Medicine, The George Washington University, Washington, DC, USA.
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA; Department of Medicine, The George Washington University, Washington, DC, USA; Department of Biomedical Engineering, The George Washington University, Washington, DC, USA.
Nanostructured Materials Laboratory, The University of Georgia, Athens, GA, USA; Department of Chemistry, The University of Georgia, Athens, GA, USA; Graduate Program of Polymer, Fibers, and Textile Sciences, USA.

We studied NE-4C neural cells differentiation on 2D polycaprolactone (PCL) nanofibrous scaffolds with systematically varied mechanical characteristics of nanofibers while retaining an unchanged fiber alignment, diameter, and chemical composition. Our experiments demonstrated that the nanofibers with enhanced mechanical properties are beneficial for the preferential development of neuronal cells vs. glial cells. Electrospun (ES) and touch-spun (TS) nanofibers were fabricated with Young's modulus in the range of 10 MPa to 230 MPa and a fraction of crystallinity from 30% to 80%. The TS fibers undergo a greater drawing ratio and thus approach a greater polymer chain stretching and alignment that resulted in an increased crystallinity. The TS scaffolds demonstrated improved stability in the aqueous cell culture environment, resisting misalignment and entanglement after a period of 2 weeks of swelling followed by 14 days of neural differentiation. The results confirmed that the neurites on the TS fibers have a preferred orientation even after swelling.

中文翻译：

机械增强的触纺纳米纤维支架增强了神经干细胞的神经元分化。

我们研究了2D聚己内酯（PCL）纳米纤维支架上NE-4C神经细胞的分化，该支架具有纳米纤维的系统变化的机械特性，同时保留了不变的纤维排列，直径和化学组成。我们的实验表明，具有增强的机械性能的纳米纤维有利于神经元细胞相对于神经胶质细胞的优先发育。制造的电纺（ES）和触纺（TS）纳米纤维的杨氏模量为10 MPa至230 MPa，结晶度分数为30％至80％。TS纤维具有更大的拉伸比，因此可以实现更大的聚合物链拉伸和排列，从而提高结晶度。TS支架在水性细胞培养环境中表现出更高的稳定性，肿胀2周，随后神经分化14天后，可以抵抗错位和纠缠。结果证实，即使在溶胀之后，TS纤维上的神经突也具有优选的取向。

更新日期：2020-01-11

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