BACKGROUND Inflammatory periodontal diseases are caused by interaction between gram negative, anaerobic bacteria and host response. Persistent infection of Pseudomonas aeruginosa in cystic fibrosis (CF) patients also cause increased pro-inflammatory response and the imbalance of pro- and anti-inflammatory response in brochoalveolar lavage fluid which leads to destruction of lungs. The aim of this study is to evaluate periodontal status of CF patients, to measure level of cytokines and biochemical molecules in gingival crevicular fluid (GCF), and to detect presence of P. aeruginosa in dental plaque samples. MATERIALS AND METHODS GCF samples were collected from 41 CF patients and 39 healthy (non-CF) subjects. Interleukin (IL)-1ß, IL-17, IL-10, human neutrophil elastase (HNE), cystic fibrosis transmembrane regulator (CFTR) protein, and human β-defensin-1 (HBD1) in GCF were evaluated by ELISA method. Dental plaque samples were collected from 18 CF patients with history of P. aeruginosa colonization and 15 non-CF subjects. Presence of P. aeruginosa was evaluated by using conventional culture methods and molecular methods. RESULTS Levels of IL-1ß, HNE, and HBD1 in CF patients were significantly higher than non-CF subjects. However, IL-10 level was significantly lower in CF patients. Increased pro-inflammatory (IL-1ß) and decreased anti-inflammatory (IL-10) cytokine levels were observed in GCF samples from CF patients, irrespective of their periodontal status. P. aeruginosa were detected in four samples of 18 CF patients, and all were negative in non-CF group. CONCLUSIONS As a result of this study, CF coexists increasing pro-inflammatory and decreasing anti-inflammatory response locally. Due to increasing pro-inflammation, CF patients should be followed-up more often than non-CF children.

中文翻译：

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囊性纤维化患者伴或不伴牙龈炎的促炎和抗炎细胞因子水平

背景炎症性牙周病是由革兰氏阴性厌氧菌和宿主反应之间的相互作用引起的。囊性纤维化 (CF) 患者中铜绿假单胞菌的持续感染也会导致支气管肺泡灌洗液中促炎反应增加和促炎反应和抗炎反应失衡，从而导致肺部破坏。本研究的目的是评估 CF 患者的牙周状况，测量龈沟液 (GCF) 中细胞因子和生化分子的水平，并检测牙菌斑样本中铜绿假单胞菌的存在。材料和方法 GCF 样品收集自 41 名 CF 患者和 39 名健康（非 CF）受试者。白细胞介素 (IL)-1ß、IL-17、IL-10、人中性粒细胞弹性蛋白酶 (HNE)、囊性纤维化跨膜调节蛋白 (CFTR)、通过ELISA方法评估GCF中的人β-防御素-1（HBD1）和人β-防御素-1（HBD1）。从具有铜绿假单胞菌定植史的 18 名 CF 患者和 15 名非 CF 受试者收集牙菌斑样本。通过使用常规培养方法和分子方法评估铜绿假单胞菌的存在。结果 CF 患者的 IL-1ß、HNE 和 HBD1 水平显着高于非 CF 受试者。然而，CF患者的IL-10水平显着降低。在 CF 患者的 GCF 样本中观察到促炎 (IL-1ß) 增加和抗炎 (IL-10) 细胞因子水平降低，无论他们的牙周状况如何。18例CF患者的4个样本中检出铜绿假单胞菌，非CF组均为阴性。结论 作为这项研究的结果，CF 共存增加促炎和减少局部抗炎反应。由于促炎症增加，CF 患者应比非 CF 儿童更频繁地进行随访。