Prostaglandins (PGs) are vitally important unsaturated fatty acids involved in arachidonic acid (AA) metabolism, participating in numerous pathophysiological processes, especially in maintaining the homeostasis of uterus. Therefore, quantitative analysis of PGs is of great importance for uncovering potential mechanisms of PGs related diseases. However, methods for determining PGs in uterine samples have not been reported. In this study, an ultra high-performance liquid chromatography/mass spectrometry (UHPLC-MS/MS) method was established to quantify PGs in uterine samples, using N,N-Dimethylethylenediamine (DMED) and N,N-Diethylethylenediamine (DEED) as derivatization reagents. The derivatization could be finished at 37 °C for 30 min catalyzed by 1-N,N,N',N'-Tetramethyl-O-(7-azabenzotriazol-1-yl) uronium hexafluorophosphate (HATU). This is a mild condition suitable for most of biological samples. The DMED labeling of PGs could significantly enhance their response compared to those of underived ones. This method exhibited excellent linearity (R2 > 0.997) and precision for the determination of PGs in uterine samples (CV ≤ 12.9%). The extraction recoveries of PGs were ranged from 83.0 to 100% and matrix effects were ranged from 86.3 to 106%, indicating DEED labeled standards could be used as internal standards for PGs quantification. With the proposed method, we successfully quantified PGs in rat uterus. The results showed their levels were significant changed in abnormal uterine bleeding (AUB) rats, suggesting that PGs might be involved in the pathological process of AUB. This established analogous reagents derivatization based UHPLC-MS/MS method could be used as a powerful tool to monitor PGs, providing insights to the precise mechanism of PG action on the endometrium.

中文翻译：

---

基于类似试剂衍生化的超高效液相色谱/质谱法定量大鼠子宫中的前列腺素。

前列腺素（PGs）是参与花生四烯酸（AA）代谢的至关重要的不饱和脂肪酸，参与许多病理生理过程，尤其是维持子宫的动态平衡。因此，对PGs进行定量分析对于揭示PGs相关疾病的潜在机制具有重要意义。然而，尚未报道确定子宫样品中PG的方法。在这项研究中，建立了一种超高效液相色谱/质谱（UHPLC-MS / MS）方法，以N，N-二甲基乙二胺（DMED）和N，N-二乙基乙二胺（DEED）为定量样品。衍生试剂。可以在1-N，N，N'，N'-四甲基-O-（7-氮杂苯并三唑-1-基）六氟磷酸铀（HATU）催化下，在37°C下完成衍生化30分钟。这是适合大多数生物样品的温和条件。PG的DMED标记与未成熟的PG相比可以显着增强它们的反应。该方法具有出色的线性（R2> 0.997）和测定子宫样品中PGs的精密度（CV≤12.9％）。PG的提取回收率范围为83.0至100％，基质效应范围为86.3至106％，表明DEED标记的标准品可用作PG定量的内标。通过提出的方法，我们成功地定量了大鼠子宫中的PG。结果表明它们的水平在异常子宫出血（AUB）大鼠中显着改变，表明PGs可能参与了AUB的病理过程。