To explain gastrodin improved cell apoptosis induced by preeclampsia in vivo and in vitro study. The PE and normal rats were injected with normal saline (Model), low‐dose gastrodin (Gas‐L), medium‐dose gastrodin (Gas‐M), and high‐dose gastrodin (Gas‐H) groups at 50, 100, or 200 mg/kg per day. The rat blood pressure and 24‐hr urine protein level were measured at pregnant days 10, 16, and 20. Evaluating pathology by H&E staining, the cell apoptosis by TUNEL, and MyD88 and NF‐κB (p65) proteins by IHC assay using H/R to simulate PE cell model. Measuring cell proliferation, apoptosis, and MyD88 and NF‐κB (p65) protein expression by MTT, flow cytometry, and WB assay. The SBP, DBP, and 24‐hr urine protein levels were significantly different in PE rats (p < .05). The SBP, DBP, and 24‐hr urine protein levels were significantly improved (p < .05) in vivo and in vitro. The positive apoptosis cells and apoptosis rate were significantly increased with MyD88 and NF‐κB (p65) proteins upregulation (p < .05). The positive apoptosis cells and apoptosis rate were significantly decreased with MyD88 and NF‐κB (p65) proteins depressing in gastrodin‐treated groups with dose‐dependent (p < .05). Gastrodin improves PE‐induced cell apoptosis and pathology changed via MyD88/NF‐κB pathway in vitro and in vivo study.

中文翻译：

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天麻素通过调节大鼠的TLR4 /NF-κB改善子痫前期诱导的细胞凋亡

解释天麻素改善先兆子痫诱导的细胞凋亡的体内和体外研究。PE和正常大鼠分别在50、100，或每天200 mg / kg。在怀孕的第10、16和20天测量大鼠的血压和24小时尿蛋白水平。通过H＆E染色评估病理学，通过TUNEL评估细胞凋亡，并通过IHC分析使用H评估MyD88和NF-κB（p65）蛋白/ R模拟PE细胞模型。通过MTT，流式细胞仪和WB测定法测量细胞增殖，凋亡以及MyD88和NF-κB（p65）蛋白表达。PE大鼠的SBP，DBP和24小时尿蛋白水平显着不同（p <.05）。 在体内和体外，SBP，DBP和24小时尿蛋白水平得到了显着改善（p <.05）。MyD88和NF-κB（p65）蛋白上调显着增加了阳性细胞的凋亡和凋亡率（p  <.05）。在剂量依赖性的天麻素治疗组中，MyD88和NF-κB（p65）蛋白抑制可显着降低阳性细胞凋亡和凋亡率（p  <.05）。天麻素通过MyD88 /NF-κB途径在体外和体内研究中改善了PE诱导的细胞凋亡和病理变化。