The supercritical fluid chromatography coupled with mass spectrometry (SFC-MS) method and liquid chromatography coupled with mass spectrometry (LC-MS) method were developed for the separation and characterization of poly (ethylene oxide) methyl glucose sesquistearate (PEO-Glu-sesquistearate). The products of PEO-Glu-sesquistearate are composed of complex oligomers. The relationship between molecular structure of these oligomers and chromatographic retention behavior in both SFC and LC were discussed and compared. As compared with LC, hydrophobic moieties of compounds favor the fast elution in SFC. The different series can be better separated by LC, while the homologues compounds in same series can be better separated by SFC, and SFC-MS provided more comprehensive structural information. Different series such as PEO-distearate, PEO-stearate, PEO, PEO-Glu-tetrastearate, PEO-Glu-tristearate, PEO-Glu-distearate, PEO-Glu-stearate, and PEO-Glu were identified by MS/MS.

中文翻译：

---

超临界流体色谱-质谱联用技术用于非离子表面活性剂的表征，并与液相色谱-质谱联用技术进行比较。

开发了超临界流体色谱-质谱联用（SFC-MS）方法和液相色谱-质谱联用（LC-MS）方法，用于分离和表征聚环氧乙烷甲基葡萄糖倍半硬脂酸酯（PEO-Glu-倍半硬脂酸酯） 。PEO-Glu-倍半硬脂酸酯的产物由复杂的低聚物组成。讨论并比较了这些低聚物的分子结构与SFC和LC中色谱保留行为之间的关系。与LC相比，化合物的疏水部分有利于SFC的快速洗脱。LC可以更好地分离不同系列，而SFC可以更好地分离相同系列中的同系物，SFC-MS提供了更全面的结构信息。不同系列，例如硬脂酸PEO，硬脂酸PEO，PEO，