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The PHD finger of Arabidopsis SIZ1 recognizes trimethylated histone H3K4 mediating SIZ1 function and abiotic stress response.
Communications Biology ( IF 5.9 ) Pub Date : 2020-01-10 , DOI: 10.1038/s42003-019-0746-2
Kenji Miura 1, 2 , Na Renhu 1 , Takuya Suzaki 1, 2
Affiliation  

Arabidopsis SIZ1 encodes a SUMO E3 ligase to regulate abiotic and biotic stress responses. Among SIZ1 or mammalian PIAS orthologs, plant SIZ1 proteins contain the plant homeodomain (PHD) finger, a C4HC3 zinc finger. Here, we investigated the importance of PHD of Arabidopsis SIZ1. The ProSIZ1::SIZ1(ΔPHD):GFP was unable to complement growth retardation, ABA hypersensitivity, and the cold-sensitive phenotype of the siz1 mutant, but ProSIZ1::SIZ1:GFP could. Substitution of C162S in the PHD finger was unable to complement the siz1 mutation. Tri-methylated histone H3K4 (H3K4me3) was recognized by PHD, not by PHD(C162S). WRKY70 was up-regulated in the siz1-2 mutant and H3K4me3 accumulated at high levels in the WRKY70 promoter. PHD interacts with ATX, which mediates methylation of histone, probably leading to suppression of ATX's function. These results suggest that the PHD finger of SIZ1 is important for recognition of the histone code and is required for SIZ1 function and transcriptional suppression.

中文翻译:

拟南芥SIZ1的PHD手指识别介导SIZ1功能和非生物应激反应的三甲基化组蛋白H3K4。

拟南芥SIZ1编码一个SUMO E3连接酶来调节非生物和生物应激反应。在SIZ1或哺乳动物PIAS直系同源物中,植物SIZ1蛋白包含植物同源域(PHD)指,即C4HC3锌指。在这里,我们调查了拟南芥SIZ1 PHD的重要性。ProSIZ1 ::: SIZ1(ΔPHD):GFP无法补充siz1突变体的生长迟缓,ABA超敏反应和冷敏感表型,但ProSIZ1 ::: SIZ1:GFP可以。PHD手指中的C162S替代不能补充siz1突变。三甲基化组蛋白H3K4(H3K4me3)被PHD识别,而不被PHD(C162S)识别。WRKY70在siz1-2突变体中上调,并且H3K4me3在WRKY70启动子中高水平积累。PHD与ATX相互作用,后者介导组蛋白的甲基化,可能导致ATX功能的抑制。
更新日期:2020-01-10
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