An enhanced cathodic electrochemiluminescence (ECL) assay for prostate-specific antigen (PSA) is developed based on the in situ activation of a semiconductor nanomaterial. An excellent ECL emitter (CdS/TiO2 nanotubes) was fabricated by the combination of TiO2 nanotubes (NTs) and thioglycolic acid-capped CdS nanocrystals (NCs). After the activation of the hydrogen peroxide-citric acid solution, the ECL signal was enhanced 265 times compared with that of the original TiO2 NT with H2O2 as co-reactant. For the ECL assay, activated CdS/TiO2 NTs were assembled with complementary DNA, PSA aptamer and probe DNA-functionalized SiO2@Pt nanoparticles (NPs) via DNA hybridization to form the detection platform. The SiO2@Pt NPs acted as ECL quencher of CdS/TiO2 NTs. In the presence of PSA, ECL increased after the release of pDNA-SiO2@Pt NPs because of the binding of PSA to the aptamer. An "off-on" ECL phenomenon appeared. The enhanced ECL signals were used for sensitive determination of PSA. The dynamic range was 0.001 to 50 ng mL-1 with a detection limit of 0.4 pg mL-1 (S/N = 3). This new approach conceivably paves the way for fabricating various other enhanced ECL emitter systems, with good application prospects in clinical practice. Graphical abstract The activated CdS/TiO2 nanotubes and SiO2@Pt nanoparticles were synthesized and used to develop an energy-transfer electrochemiluminescence analysis method with high sensitivity and anti-interference performance.

中文翻译：

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载有CdS纳米晶体的TiO2纳米管作为增强的电致发光体：在前列腺特异性抗原检测中的应用。

基于半导体纳米材料的原位活化，开发了一种针对前列腺特异性抗原（PSA）的增强型阴极电化学发光（ECL）分析方法。通过将TiO2纳米管（NTs）和巯基乙酸封端的CdS纳米晶体（NCs）结合，制得了优异的ECL发射极（CdS / TiO2纳米管）。活化过氧化氢柠檬酸溶液后，ECL信号比起原始的TiO2 NT和H2O2作为共反应物，增强了265倍。对于ECL分析，将活化的CdS / TiO2 NTs与互补DNA，PSA适体组装在一起，并通过DNA杂交探测DNA功能化的SiO2 @ Pt纳米颗粒（NPs）以形成检测平台。SiO2 @ Pt NPs作为CdS / TiO2 NTs的ECL猝灭剂。在有PSA的情况下，释放pDNA-SiO2 @ Pt NP后，ECL增加，因为PSA与适体结合。出现“关闭” ECL现象。增强的ECL信号用于PSA的灵敏测定。动态范围为0.001至50 ng mL-1，检测极限为0.4 pg mL-1（S / N = 3）。可以想象，这种新方法为制造各种其他增强型ECL发射器系统铺平了道路，在临床实践中具有良好的应用前景。摘要合成了活化的CdS / TiO2纳米管和SiO2 @ Pt纳米颗粒，用于开发具有高灵敏度和抗干扰性能的能量转移电化学发光分析方法。动态范围为0.001至50 ng mL-1，检测极限为0.4 pg mL-1（S / N = 3）。可以想象，这种新方法为制造各种其他增强型ECL发射器系统铺平了道路，在临床实践中具有良好的应用前景。摘要合成了活化的CdS / TiO2纳米管和SiO2 @ Pt纳米颗粒，用于开发具有高灵敏度和抗干扰性能的能量转移电化学发光分析方法。动态范围为0.001至50 ng mL-1，检测极限为0.4 pg mL-1（S / N = 3）。可以想象，这种新方法为制造各种其他增强型ECL发射器系统铺平了道路，在临床实践中具有良好的应用前景。摘要合成了活化的CdS / TiO2纳米管和SiO2 @ Pt纳米粒子，用于开发具有高灵敏度和抗干扰性能的能量转移电化学发光分析方法。