INTRODUCTION The abuse of anabolic androgenic steroids (AASs) is a source of public concern because of their adverse effects. Supratherapeutic doses of AASs are known to be hepatotoxic and regulate the lipoproteins in plasma by modifying the metabolism of lipids in the liver, which is associated with metabolic diseases. However, the effect of AASs on the profile of lipids in plasma is unknown. OBJECTIVES To describe the changes in the plasma lipidome exerted by AASs and to discuss these changes in the light of previous research about AASs and de novo lipogenesis in the liver. METHODS We treated male Wistar rats with supratherapeutic doses of nandrolone decanoate and testosterone undecanoate. Subsequently, we isolated the blood plasma and performed lipidomics analysis by liquid chromatography-high resolution mass spectrometry. RESULTS Lipid profiling revealed a decrease of sphingolipids and glycerolipids with palmitic, palmitoleic, stearic, and oleic acids. In addition, lipid profiling revealed an increase in free fatty acids and glycerophospholipids with odd-numbered chain fatty acids and/or arachidonic acid. CONCLUSION The lipid profile presented herein reports the imprint of AASs on the plasma lipidome, which mirrors the downregulation of de novo lipogenesis in the liver. In a broader perspective, this profile will help to understand the influence of androgens on the lipid metabolism in future studies of diseases with dysregulated lipogenesis (e.g. type 2 diabetes, fatty liver disease, and hepatocellular carcinoma).

中文翻译：

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合成代谢雄激素类固醇对血浆脂质组进行选择性重塑，反映出肝脏中从头脂肪形成的减少。

简介合成代谢雄激素类固醇（AASs）的滥用由于其不利影响而引起了公众的关注。已知AAS的超治疗剂量具有肝毒性，并通过改变肝脏中与代谢疾病相关的脂质的代谢来调节血浆中的脂蛋白。但是，AAS对血浆中脂质分布的影响尚不清楚。目的描述AAS引起的血浆脂质组的变化，并根据先前有关AAS和肝脏新生脂肪形成的研究来讨论这些变化。方法我们用超剂量的癸酸诺龙和十一酸睾丸酮治疗雄性Wistar大鼠。随后，我们分离了血浆并通过液相色谱-高分辨率质谱法进行了脂质组学分析。结果脂质分布图显示，棕榈酸，棕榈油酸，硬脂酸和油酸降低了鞘脂和甘油脂。另外，脂质谱分析显示游离脂肪酸和甘油磷脂与奇数链脂肪酸和/或花生四烯酸的含量增加。结论本文介绍的脂质谱报道了AAS在血浆脂质组上的印记，这反映了肝脏新生脂质生成的下调。从更广泛的角度来看，这种概况将有助于在未来脂代谢异常疾病（例如2型糖尿病，脂肪肝和肝细胞癌）的研究中了解雄激素对脂质代谢的影响。脂质谱分析显示，带有奇数链脂肪酸和/或花生四烯酸的游离脂肪酸和甘油磷脂增加。结论本文介绍的脂质谱报道了AAS在血浆脂质组上的印记，这反映了肝脏新生脂质生成的下调。从更广泛的角度来看，这种概况将有助于在未来脂代谢异常疾病（例如2型糖尿病，脂肪肝和肝细胞癌）的研究中了解雄激素对脂质代谢的影响。脂质谱分析显示，带有奇数链脂肪酸和/或花生四烯酸的游离脂肪酸和甘油磷脂增加。结论本文介绍的脂质谱报道了AAS在血浆脂质组上的印记，这反映了肝脏新生脂质生成的下调。从更广泛的角度来看，这种概况将有助于在未来脂代谢异常疾病（例如2型糖尿病，脂肪肝和肝细胞癌）的研究中了解雄激素对脂质代谢的影响。