Biological synthesis of nanoparticles is a growing research trend because it has numerous applications in pharmaceutics and biomedicine. The aim of this study was to obtain silver nanoparticles (AgNPs) from Bacillus sp. KFU36, a marine strain, and to assess its anticancer activity. The supernatant of Bacillus sp. KFU36 was supplemented with silver nitrate and the nanoparticles obtained were characterized spectrophotometrically and microscopically. A band of surface plasmon resonance was appeared at 430 nm, as revealed by UV-vis spectrophotometry. X-ray diffraction spectrum and Energy Dispersive Spectroscopy confirmed the crystalline and metallic structure of the AgNPs, respectively. Scanning electron microscopy revealed that the shape of the synthesized AgNPs were spherical and the size extended between 5 and 15 nm. The AgNPs were investigated for their potential anticancer effects on the cell viability, migration and apoptosis using MTT and wound-healing assays, and flow cytometry, respectively. The cytotoxic effects of these nanoparticles were evidenced by the decreasing the cell viability (as 15% at 50 μg/ml), cell density, adhesion capacity and losing the normal shape and size, and inducing the apoptosis on MCF-7 by 61% at 50 μg/ml. These findings confirm that the synthesized AgNPs exhibited superior anticancer activities and therefore could be exploited as a promising, cost-effective, and environmentally benign strategy in treating this disease in future.

中文翻译：

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芽孢杆菌KFU36合成的银纳米颗粒及其通过诱导凋亡机制对乳腺癌MCF-7细胞的抗癌作用。

纳米颗粒的生物合成是一个日益增长的研究趋势，因为它在制药和生物医学中具有许多应用。这项研究的目的是从芽孢杆菌中获得银纳米颗粒（AgNPs）。KFU36，一种海洋菌株，并评估其抗癌活性。芽孢杆菌属的上清液。KFU36补充有硝酸银，并用分光光度法和显微镜对得到的纳米颗粒进行了表征。如UV-可见分光光度法所揭示的，在430nm处出现表​​面等离振子共振带。X射线衍射光谱和能量分散光谱分别证实了AgNP的晶体和金属结构。扫描电子显微镜显示，合成的AgNP的形状为球形，大小在5至15nm之间。分别使用MTT和伤口愈合试验以及流式细胞仪研究了AgNP对细胞活力，迁移和凋亡的潜在抗癌作用。这些纳米颗粒的细胞毒性作用通过降低细胞活力（在50μg/ ml时为15％），细胞密度，黏附能力和失去正常形状和大小，并在60℃时诱导MCF-7凋亡减少61％来证明。 50微克/毫升 这些发现证实，合成的AgNPs显示出优异的抗癌活性，因此可以被用作未来治疗该疾病的有希望的，成本有效的和环境友好的策略。这些纳米颗粒的细胞毒性作用通过降低细胞活力（在50μg/ ml时为15％），细胞密度，黏附能力和失去正常形状和大小，并在60℃时诱导MCF-7凋亡减少61％来证明。 50微克/毫升 这些发现证实，合成的AgNPs显示出优异的抗癌活性，因此可以被用作未来治疗该疾病的有希望的，成本有效的和环境友好的策略。这些纳米颗粒的细胞毒性作用通过降低细胞活力（在50μg/ ml时为15％），细胞密度，黏附能力和失去正常形状和大小，并在60℃时诱导MCF-7凋亡减少61％来证明。 50微克/毫升 这些发现证实，合成的AgNPs表现出优异的抗癌活性，因此可以被用作未来治疗该疾病的有希望，具有成本效益和环境友好的策略。