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Novel Bacterial Diversity and Fragmented eDNA Identified in Hyperbiofilm-Forming Pseudomonas aeruginosa Rugose Small Colony Variant.
iScience ( IF 5.8 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.isci.2020.100827
Binbin Deng 1 , Subhadip Ghatak 2 , Subendu Sarkar 2 , Kanhaiya Singh 2 , Piya Das Ghatak 2 , Shomita S Mathew-Steiner 2 , Sashwati Roy 2 , Savita Khanna 2 , Daniel J Wozniak 3 , David W McComb 4 , Chandan K Sen 5
Affiliation  

Pseudomonas aeruginosa biofilms represent a major threat to health care. Rugose small colony variants (RSCV) of P. aeruginosa, isolated from chronic infections, display hyperbiofilm phenotype. RSCV biofilms are highly resistant to antibiotics and host defenses. This work shows that RSCV biofilm aggregates consist of two distinct bacterial subpopulations that are uniquely organized displaying contrasting physiological characteristics. Compared with that of PAO1, the extracellular polymeric substance of RSCV PAO1ΔwspF biofilms presented unique ultrastructural characteristics. Unlike PAO1, PAO1ΔwspF released fragmented extracellular DNA (eDNA) from live cells. Fragmented eDNA, thus released, was responsible for resistance of PAO1ΔwspF biofilm to disruption by DNaseI. When added to PAO1, such fragmented eDNA enhanced biofilm formation. Disruption of PAO1ΔwspF biofilm was achieved by aurine tricarboxylic acid, an inhibitor of DNA-protein interaction. This work provides critical novel insights into the contrasting structural and functional characteristics of a hyperbiofilm-forming clinical bacterial variant relative to its own wild-type strain.



中文翻译:

新型细菌多样性和片段化的eDNA在形成超生物膜的铜绿假单胞菌Rugose小菌落变体中鉴定。

铜绿假单胞菌生物膜代表了对卫生保健的主要威胁。有皱纹的小菌落变体的(RSCV)P。从慢性感染中分离出来的铜绿显示超生物膜表型。RSCV生物膜对抗生素和宿主防御具有高度抗性。这项工作表明,RSCV生物膜聚集体由两个截然不同的细菌亚群组成,它们的独特组织显示出不同的生理特征。与PAO1相比,RSCVPAO1ΔwspF生物膜的细胞外聚合物呈现出独特的超微结构特征。与PAO1不同,PAO1ΔwspF从活细胞中释放出碎片化的细胞外DNA(eDNA)。如此释放的片段化eDNA负责PAO1ΔwspF生物膜对DNaseI破坏的抗性。当添加到PAO1中时,这种片段化的eDNA增强了生物膜的形成。PAO1ΔwspF生物膜的破坏是通过金黄色三羧酸(一种DNA-蛋白质相互作用的抑制剂)实现的。这项工作提供了关键的新见解,形成相对于其自身的野生型菌株的形成超生物膜的临床细菌变异体的对比结构和功能特征。

更新日期:2020-01-09
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