Most metazoan cells entering mitosis undergo characteristic rounding, which is important for accurate spindle positioning and chromosome separation. Rounding is driven by contractile tension generated by myosin motors in the sub-membranous actin cortex. Recent studies highlight that alongside myosin activity, cortical actin organization is a key regulator of cortex tension. Yet, how mitotic actin organization is controlled remains poorly understood. To address this, we characterized the F-actin interactome in spread interphase and round mitotic cells. Using super-resolution microscopy, we then screened for regulators of cortex architecture and identified the intermediate filament vimentin and the actin-vimentin linker plectin as unexpected candidates. We found that vimentin is recruited to the mitotic cortex in a plectin-dependent manner. We then showed that cortical vimentin controls actin network organization and mechanics in mitosis and is required for successful cell division in confinement. Together, our study highlights crucial interactions between cytoskeletal networks during cell division.

中文翻译：

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F-肌动蛋白相互作用组揭示波形蛋白是有丝分裂中肌动蛋白组织和细胞力学的关键调节因子。

进入有丝分裂的大多数后生细胞会经历特征性的舍入，这对于精确的纺锤体定位和染色体分离很重要。舍入是由膜下肌动蛋白皮层中肌球蛋白马达产生的收缩张力驱动的。最近的研究强调，除了肌球蛋白活性外，皮层肌动蛋白组织是皮层张力的关键调节器。然而，如何控制有丝分裂肌动蛋白的组织仍然知之甚少。为了解决这个问题，我们表征了F-肌动蛋白相互作用组在扩散的相间和圆形有丝分裂细胞中。然后，使用超高分辨率显微镜，我们筛选了皮质结构的调节剂，并确定了中间丝波形蛋白和肌动蛋白波形蛋白连接蛋白凝集素是意料之外的候选物。我们发现波形蛋白以凝集素依赖性方式被募集到有丝分裂皮质。然后，我们表明皮质波形蛋白控制有丝分裂中的肌动蛋白网络组织和力学，并且是成功进行细胞分裂所必需的。总之，我们的研究突出了细胞分裂过程中细胞骨架网络之间的关键相互作用。