Physiological functions depend on a coordinated interplay of numerous different cell types. Proteins serve as major signaling molecules between cells; however, their comprehensive investigation in physiologically relevant settings has remained challenging. Mass spectrometry (MS)-based shotgun proteomics is emerging as a powerful technology for the systematic analysis of protein-mediated intercellular signaling and regulated post-translational modifications. Here, we discuss recent advancements in cell biological, chemical, and biochemical MS-based approaches for the profiling of cellular messengers released by sending cells, receptors expressed on the cell surface, and their interactions. We highlight methods tailored toward the mapping of dynamic signal transduction mechanisms at cellular interfaces and approaches to dissect communication cell specifically in heterocellular systems. Thereby, MS-based proteomics contributes a unique systems biology perspective for the identification of intercellular signaling pathways deregulated in disease.

中文翻译：

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用基于质谱的蛋白质组学解剖细胞间信号。

生理功能取决于许多不同细胞类型的协调相互作用。蛋白质是细胞之间的主要信号分子。然而，他们在生理相关环境中的全面调查仍然具有挑战性。基于质谱（MS）的shot弹枪蛋白质组学是一种功能强大的技术，可用于系统分析蛋白质介导的细胞间信号传导和调控的翻译后修饰。在这里，我们讨论了基于细胞生物学，化学和生化MS的方法的最新进展，这些方法用于分析发送细胞释放的细胞信使，在细胞表面表达的受体及其相互作用。我们重点介绍了针对动态信号转导机制在细胞界面上进行映射的方法，以及专门用于在异细胞系统中解剖通信细胞的方法。因此，基于MS的蛋白质组学为识别疾病中失调的细胞间信号通路提供了独特的系统生物学视角。