当前位置: X-MOL 学术Food Chem. Toxicol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing.
Food and Chemical Toxicology ( IF 4.3 ) Pub Date : 2020-01-10 , DOI: 10.1016/j.fct.2020.111125
Suramya Waidyanatha 1 , Jessica Pierfelice 2 , Tim Cristy 2 , Esra Mutlu 1 , Brian Burback 2 , Cynthia V Rider 1 , Kristen Ryan 1
Affiliation  

Botanical dietary supplements (BDS) are used around the world for many purported therapeutic properties. The selection of an authentic product and it's phytochemical characterization is critical to generate robust safety data. Because botanicals are complex mixtures with variable quality, identification of a representative product for testing has been challenging. Echinacea is used for its purported immune stimulant properties and was listed as the 2nd top-selling BDS in 2018. However, there are limited safety data for Echinacea. Hence, the National Toxicology Program (NTP) has selected Echinacea for safety testing using rodent models. Here, we describe selection and comprehensive characterization of an Echinacea purpurea root extract to be used in the NTP testing program. Using non-targeted chemical analyses combined with chemometric analysis, a potential unfinished product (i.e., an extract that serves as source material for finished products) of Echinacea purpurea was selected. The product was then authenticated using chemical and DNA techniques and characterized, including the phytochemical composition. Among numerous constituents identified, caftaric acid, chicoric acid, chlorogenic acid and dodeca-2(E),4(E),8(Z),10(E/Z)-tetraenoic acid isobutylamide made up a small fraction of the extract. Based on these analyses, an approach is proposed for test article selection for Echinacea research which can be adapted to other botanicals.

中文翻译:

一种用于安全性测试的紫锥菊提取物的测试物品选择和植物化学表征策略。

植物性膳食补充剂(BDS)在世界范围内具有许多声称的治疗特性。选择可靠的产品及其植物化学特征对于生成可靠的安全数据至关重要。由于植物药是质量可变的复杂混合物,因此鉴定要测试的代表性产品一直具有挑战性。紫锥菊因其声称的免疫刺激特性而被使用,并在2018年被列为第二大畅销BDS。但是,紫锥菊的安全性数据有限。因此,美国国家毒理学计划(NTP)已选择紫锥菊用于使用啮齿动物模型进行安全性测试。在这里,我们描述了在NTP测试程序中使用的紫锥菊紫锥菊根提取物的选择和全面表征。结合使用非目标化学分析和化学计量分析,选择了紫锥菊的潜在未加工产品(即用作最终产品原料的提取物)。然后使用化学和DNA技术对产品进行身份验证,并对其特征进行鉴定,包括植物化学成分。在鉴定出的许多成分中,咖啡酸,癸二酸,绿原酸和十二碳2(E),4(E),8(Z),10(E / Z)-丁烯酸异丁酰胺占萃取物中的一小部分。在这些分析的基础上,提出了一种适用于紫锥菊研究的测试样品选择方法,该方法可以适用于其他植物药。包括植物化学成分。在鉴定出的许多成分中,咖啡酸,癸二酸,绿原酸和十二碳2(E),4(E),8(Z),10(E / Z)-丁烯酸异丁酰胺占萃取物中的一小部分。在这些分析的基础上,提出了一种适用于紫锥菊研究的测试样品选择方法,该方法可以适用于其他植物药。包括植物化学成分。在鉴定出的许多成分中,咖啡酸,癸二酸,绿原酸和十二碳2(E),4(E),8(Z),10(E / Z)-丁烯酸异丁酰胺占萃取物中的一小部分。在这些分析的基础上,提出了一种适用于紫锥菊研究的测试样品选择方法,该方法可以适用于其他植物药。
更新日期:2020-01-11
down
wechat
bug