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Inhalation of welding fumes reduced sperm counts and high fat diet reduced testosterone levels; differential effects in Sprague Dawley and Brown Norway rats.
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-01-10 , DOI: 10.1186/s12989-019-0334-0 Astrid Skovmand 1, 2 , Aaron Erdely 3, 4, 5 , James M Antonini 3, 5 , Timothy R Nurkiewicz 3, 4, 5 , Mohammad Shoeb 3 , Tracy Eye 3 , Vamsi Kodali 3 , Katrin Loeschner 6 , Janja Vidmar 6 , Jørgen S Agerholm 2 , Sandra Goericke-Pesch 7 , Ulla Vogel 1, 8 , Karin S Hougaard 1, 9
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-01-10 , DOI: 10.1186/s12989-019-0334-0 Astrid Skovmand 1, 2 , Aaron Erdely 3, 4, 5 , James M Antonini 3, 5 , Timothy R Nurkiewicz 3, 4, 5 , Mohammad Shoeb 3 , Tracy Eye 3 , Vamsi Kodali 3 , Katrin Loeschner 6 , Janja Vidmar 6 , Jørgen S Agerholm 2 , Sandra Goericke-Pesch 7 , Ulla Vogel 1, 8 , Karin S Hougaard 1, 9
Affiliation
Previous studies have shown that inhalation of welding fumes may induce pulmonary and systemic inflammation and organ accumulation of metal, to which spermatogenesis and endocrine function may be sensitive. Also obesity may induce low-grade systemic inflammation. This study aimed to investigate the effects on sperm production of inhaled metal nanoparticles from stainless steel welding, and the potential exacerbation by intake of a high fat diet. Both the inbred Brown Norway and the outbred Sprague Dawley rat strains were included to study the influence of strain on the detection of toxicity. Rats were fed regular or high fat (HF) diet for 24 weeks and were exposed to 20 mg/m3 of gas metal arc-stainless steel (GMA-SS) welding fumes or filtered air for 3 h/day, 4 days/week for 5 weeks, during weeks 7–12. Outcomes were assessed upon termination of exposure (week 12) and after recovery (week 24). At week 12, the GMA-SS exposure induced pulmonary inflammation in both strains, without consistent changes in markers of systemic inflammation (CRP, MCP-1, IL-6 and TNFα). GMA-SS exposure lowered daily sperm production compared to air controls in Sprague Dawley rats, but only in GMA-SS Brown Norway rats also fed the HF diet. Overall, HF diet rats had lower serum testosterone levels compared to rats on regular diet. Metal content in the testes was assessed in a limited number of samples in Brown Norway rats, but no increase was obsedrved. At week 24, bronchoalveolar lavage cell counts had returned to background levels for GMA-SS exposed Sprague Dawley rats but remained elevated in Brown Norway rats. GMA-SS did not affect daily sperm production statistically significantly at this time point, but testicular weights were lowered in GMA-SS Sprague Dawley rats. Serum testosterone remained lowered in Sprague Dawley rats fed the HF diet. Exposure to GMA-SS welding fumes lowered sperm production in two strains of rats, whereas high fat diet lowered serum testosterone. The effect on sperm counts was likely not mediated by inflammation or lowered testosterone levels. The studied reproductive outcomes seemed more prone to disruption in the Sprague Dawley compared to the Brown Norway strain.
中文翻译:
吸入焊接烟雾可减少精子数量,高脂饮食可降低睾丸激素水平;在Sprague Dawley和Brown Norway大鼠中的差异作用。
先前的研究表明,吸入焊接烟尘可能会引起肺部和全身炎症以及金属器官的积聚,精子发生和内分泌功能可能对金属敏感。肥胖也可能诱发轻度全身性炎症。这项研究旨在研究不锈钢焊接对吸入金属纳米颗粒的精子产生的影响,以及高脂饮食的摄入可能加剧这种影响。包括自交系的Brown Norway和Sprague Dawley自交系的大鼠菌株,以研究菌株对毒性检测的影响。给大鼠喂食常规或高脂(HF)饮食24周,并使其暴露于20 mg / m3的气体金属电弧不锈钢(GMA-SS)焊接烟气或过滤空气中,持续3 h /天,4天/周。 5周,7-12周。在暴露结束后(第12周)和恢复后(第24周)评估结局。在第12周时,GMA-SS暴露在两种菌株中均引起了肺部炎症,而全身性炎症标志物(CRP,MCP-1,IL-6和TNFα)却没有发生一致的变化。与空气对照组相比,在Sprague Dawley大鼠中,GMA-SS暴露降低了每日精子产量,但仅在GMA-SS Brown挪威大鼠中也饲喂了HF饮食。总体而言,与常规饮食相比,HF饮食大鼠的血清睾丸激素水平较低。在棕色挪威大鼠中,在有限数量的样品中评估了睾丸中的金属含量,但没有发现增加。在第24周时,暴露于GMA-SS的Sprague Dawley大鼠的支气管肺泡灌洗细胞计数已恢复至本底水平,但在Brown Norway大鼠中仍然升高。在这个时间点,GMA-SS并未显着影响每日精子的产生,但GMA-SS Sprague Dawley大鼠的睾丸重量降低了。喂养HF饮食的Sprague Dawley大鼠的血清睾丸激素水平仍然降低。暴露于GMA-SS焊接烟气会降低两只大鼠的精子产生,而高脂饮食会降低血清睾丸激素。对精子数量的影响可能不是由炎症或睾丸激素水平降低所介导的。与布朗挪威菌株相比,Sprague Dawley研究的生殖结果似乎更容易受到干扰。而高脂饮食降低了血清睾丸激素。对精子数量的影响可能不是由炎症或睾丸激素水平降低所介导的。与布朗挪威菌株相比,Sprague Dawley研究的生殖结果似乎更容易受到干扰。而高脂饮食降低了血清睾丸激素。对精子数量的影响可能不是由炎症或睾丸激素水平降低所介导的。与布朗挪威菌株相比,Sprague Dawley研究的生殖结果似乎更容易受到干扰。
更新日期:2020-01-10
中文翻译:
吸入焊接烟雾可减少精子数量,高脂饮食可降低睾丸激素水平;在Sprague Dawley和Brown Norway大鼠中的差异作用。
先前的研究表明,吸入焊接烟尘可能会引起肺部和全身炎症以及金属器官的积聚,精子发生和内分泌功能可能对金属敏感。肥胖也可能诱发轻度全身性炎症。这项研究旨在研究不锈钢焊接对吸入金属纳米颗粒的精子产生的影响,以及高脂饮食的摄入可能加剧这种影响。包括自交系的Brown Norway和Sprague Dawley自交系的大鼠菌株,以研究菌株对毒性检测的影响。给大鼠喂食常规或高脂(HF)饮食24周,并使其暴露于20 mg / m3的气体金属电弧不锈钢(GMA-SS)焊接烟气或过滤空气中,持续3 h /天,4天/周。 5周,7-12周。在暴露结束后(第12周)和恢复后(第24周)评估结局。在第12周时,GMA-SS暴露在两种菌株中均引起了肺部炎症,而全身性炎症标志物(CRP,MCP-1,IL-6和TNFα)却没有发生一致的变化。与空气对照组相比,在Sprague Dawley大鼠中,GMA-SS暴露降低了每日精子产量,但仅在GMA-SS Brown挪威大鼠中也饲喂了HF饮食。总体而言,与常规饮食相比,HF饮食大鼠的血清睾丸激素水平较低。在棕色挪威大鼠中,在有限数量的样品中评估了睾丸中的金属含量,但没有发现增加。在第24周时,暴露于GMA-SS的Sprague Dawley大鼠的支气管肺泡灌洗细胞计数已恢复至本底水平,但在Brown Norway大鼠中仍然升高。在这个时间点,GMA-SS并未显着影响每日精子的产生,但GMA-SS Sprague Dawley大鼠的睾丸重量降低了。喂养HF饮食的Sprague Dawley大鼠的血清睾丸激素水平仍然降低。暴露于GMA-SS焊接烟气会降低两只大鼠的精子产生,而高脂饮食会降低血清睾丸激素。对精子数量的影响可能不是由炎症或睾丸激素水平降低所介导的。与布朗挪威菌株相比,Sprague Dawley研究的生殖结果似乎更容易受到干扰。而高脂饮食降低了血清睾丸激素。对精子数量的影响可能不是由炎症或睾丸激素水平降低所介导的。与布朗挪威菌株相比,Sprague Dawley研究的生殖结果似乎更容易受到干扰。而高脂饮食降低了血清睾丸激素。对精子数量的影响可能不是由炎症或睾丸激素水平降低所介导的。与布朗挪威菌株相比,Sprague Dawley研究的生殖结果似乎更容易受到干扰。