BACKGROUND Incidence of anal and oral infections with Human Papillomavirus (HPV) is increasing, particularly among Human Immunodeficiency Virus-positive (HIV+) men. HPV type 16 has exhibited the highest incidence and only limited data is available on other prevalent types, variants of HPV16, as well as associated factors. We were interested in identifying prevalent HPV types, variants of type 16, as well as factors associated with HPV16 infections in the oral cavity of HIV+ men who have sex with men (MSM). METHODS A cross-sectional study of oral cavity samples from HIV+ MSM, that in a previous study were identified as positive for HPV16 in the anal canal. Cells from the oral cavity (102 samples, paired with 102 from the anal canal of same patient) were used to extract DNA and detect HPV infections using INNO-LiPA HPV Genotyping Extra II, and PCR. From these, 80 samples (paired, 40 anal and 40 oral) were used to identify variants of type 16 by sequencing. Statistical differences were estimated by the X2 test, and p values equal to or less than 0.05 were considered significant. SPSS ver. Twenty-four statistical software (IBM Corp) was used. RESULTS We found a high prevalence of High-Risk HPV (HR-HPV) and Low-Risk HPV (LR-HPV). Patients were positive in the oral cavity for HR types; 16, 39 and 18 (80.4, 61.8 and 52.9% respectively) and LR types 11 and 6 (53.9 and 34.3% respectively). Surprisingly, only European variants of type 16 were found in the oral cavity, although American Asian (22.5%) and African (2.5%) variants were identified in the anal canal. The analysis showed that CD4 counts could be the most important risk factor associated with HR-HPV infections in the oral cavity, anal canal or both anatomical regions. The risk of infection of the oral cavity with type 18 increased in men diagnosed with HIV for more than 6 years. CONCLUSIONS Prevalence of both HR and LR HPV's in the oral cavity of Mexican HIV+ MSM is very high. The fact that only European variants of HPV16 were found in the oral cavity suggest a possible tropism not previously described.

中文翻译：

---

无症状HIV阳性男性口腔中高度流行的人乳头瘤病毒感染。

背景技术人乳头瘤病毒（HPV）的肛门和口腔感染的发生率正在增加，尤其是在人类免疫缺陷病毒阳性（HIV +）男性中。HPV 16型的发病率最高，只有关于其他流行类型，HPV16的变体以及相关因素的有限数据。我们对确定流行的HPV类型，16型变异以及与男男性接触者（MSM）的HIV +男性口腔中HPV16感染相关的因素感兴趣。方法一项来自HIV + MSM口腔样品的横断面研究在先前的研究中被鉴定为肛管内HPV16阳性。使用INNO-LiPA HPV基因分型Extra II和PCR，将来自口腔的细胞（102个样本，与同一位患者的肛管中的102个配对）用于提取DNA并检测HPV感染。从这些样本中，使用80个样品（配对，40个肛门和40个口服）通过测序鉴定16型变体。通过X2检验估计统计差异，并且p值等于或小于0.05被认为是显着的。SPSS版本 使用了二十四个统计软件（IBM Corp）。结果我们发现高风险HPV（HR-HPV）和低风险HPV（LR-HPV）的患病率很高。患者口腔中的HR类型阳性；16、39和18（分别为80.4、61.8和52.9％）和LR类型11和6（分别为53.9和34.3％）。令人惊讶的是，尽管在肛门管中发现了美洲亚洲人（22.5％）和非洲人（2.5％）变体，但在口腔中仅发现了欧洲16型变体。分析表明，CD4计数可能是与口腔中HR-HPV感染相关的最重要的危险因素，肛管或两个解剖区域。被诊断患有HIV的男性超过6年的人，感染18型口腔的风险增加。结论墨西哥HIV + MSM口腔中HR和LR HPV的患病率很高。在口腔中仅发现HPV16的欧洲变种这一事实表明，以前没有描述过这种可能的向性。