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DNA demethylation is involved in the regulation of temperature-dependent anthocyanin accumulation in peach.
The Plant Journal ( IF 7.2 ) Pub Date : 2020-01-10 , DOI: 10.1111/tpj.14680
Yong-Chao Zhu 1 , Bo Zhang 1 , Andrew C Allan 2, 3 , Kui Lin-Wang 2 , Yun Zhao 1 , Ke Wang 1 , Kun-Song Chen 1 , Chang-Jie Xu 1
Affiliation  

Anthocyanin biosynthesis is induced by low temperatures in a number of plants. However, in peach (cv Zhonghuashoutao), anthocyanin accumulation was observed in fruit stored at 16°C but not at or below 12°C. Fruit stored at 16°C showed elevated transcript levels of genes encoding anthocyanin biosynthetic enzymes, the transport protein glutathione S-transferase and key transcription factors. Higher transcript levels of PpPAL1/2, PpC4H, Pp4CL4/5/8, PpF3H, PpF3'H, PpDFR1/2/3 and PpANS, as well as transcription factor gene PpbHLH3, were associated with lower methylation levels in the promoter of these genes. The DNA methylation level was further highly correlated with the expression of the DNA methyltransferase genes and DNA demethylase genes. The application of DNA methylation inhibitor 5-azacytidine induced anthocyanin accumulation in peach flesh, further implicating a critical role for DNA demethylation in regulating anthocyanin accumulation in peach flesh. Our data reveal that temperature-dependent DNA demethylation is a key factor to the post-harvest temperature-dependent anthocyanin accumulation in peach flesh.

中文翻译:

DNA脱甲基化参与调节桃子中温度依赖性花色苷的积累。

在许多植物中,低温诱导花色苷的生物合成。然而,在桃子中,在16°C而不是12°C或低于12°C的水果中观察到花青素积累。储存在16°C的水果显示出编码花色苷生物合成酶,转运蛋白谷胱甘肽S-转移酶和关键转录因子的基因的转录水平升高。PpPAL1 / 2,PpC4H,Pp4CL4 / 5/8,PpF3H,PpF3'H,PpDFR1 / 2/3和PpANS的较高转录水平以及转录因子基因PpbHLH3与这些基因启动子中较低的甲​​基化水平相关。DNA甲基化水平进一步与DNA甲基转移酶基因和DNA脱甲基酶基因的表达高度相关。DNA甲基化抑制剂5-氮杂胞苷在桃肉中花色苷积累的应用 进一步暗示了DNA去甲基化在调节桃肉中花色苷积累中的关键作用。我们的数据表明,温度依赖性DNA去甲基化是桃果肉收获后温度依赖性花色苷积累的关键因素。
更新日期:2020-01-10
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