Abstract A novel nanoacetylene black coated the surface of graphite pencil lead electrode (nano-AB/GPLE) is successfully constructed. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) indicated that the new designed nano-AB/GPLE possessed a fast electron transfer compared with the bare GPLE and enhanced electrochemical response toward electroreduction of antiandrogens flutamide (FLU) and cyproterone acetate (CPA) simultaneously. From the nano-AB/GPLE, two well separated reduction signals of coexistence FLU and CPA at nano-AB/GPLE were obtained. Electrochemical sensing of FLU and CPA in the presence of the physiological common interferents at nano-AB/GPLE was examined and confirmed the selectivity for the detection analysis of the two antiandrogens without cross interference of biological compounds. The limits of detection for coexistence of FLU and CPA are 1.39 nM and 4.74 nM, respectively. Finally, the applicability of the designed sensor is performed for detection of the two antiandrogens FLU and CPA simultaneously in pharmaceutical tablets and biological fluids with good accuracy and precision.

中文翻译：

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基于纳米乙炔黑改性石墨铅笔铅电极表面的新型一次性电化学传感器同时测定抗雄激素氟他胺和醋酸环丙孕酮

摘要 成功构建了一种新型纳米乙炔黑包覆石墨铅笔铅电极（nano-AB/GPLE）。电化学阻抗谱 (EIS) 和循环伏安法 (CV) 表明，与裸 GPLE 相比，新设计的纳米 AB/GPLE 具有快速的电子转移，并增强了对抗雄激素氟他胺 (FLU) 和醋酸环丙孕酮 (CPA) 电还原的电化学响应同时地。从nano-AB/GPLE 中，获得了nano-AB/GPLE 共存FLU 和CPA 的两个分离良好的还原信号。在纳米 AB/GPLE 存在生理学常见干扰物的情况下，对 FLU 和 CPA 的电化学传感进行了检查，并证实了两种抗雄激素检测分析的选择性，而没有生物化合物的交叉干扰。FLU 和 CPA 共存的检测限分别为 1.39 nM 和 4.74 nM。最后，设计的传感器适用于同时检测药片和生物流体中的两种抗雄激素 FLU 和 CPA，具有良好的准确度和精密度。