Abstract In this paper, a new type of magnetic thermosensitive fluorescence imprinted polymer was prepared for high selective identification of bovine hemoglobin with the magnetic carbon dots as the fluorescence source, poly(n-isopropylamide) and N, N-methylenebis acrylamide as the functional monomers and cross-linker, respectively. The magnetic thermosensitive fluorescence imprinted polymer was characterized by Fourier-transform infrared, fluorescence spectrometer, scanning electron microscope, transmission electron microscope and vibrating sample magnetometer in detail. The fluorescence of the magnetic thermosensitive fluorescence imprinted polymer was quenched linearly with bovine hemoglobin concentration in the range of 0.1- 9.0 μM with the detection limit of 48.0 nM. The imprinting factor was 2.3, which indicated excellent specificity of the magnetic thermosensitive fluorescence imprinted polymer toward bovine hemoglobin. With the advantage of the thermosensitivity, the elution efficiency of bovine hemoglobin could be significantly simplified and enhanced. In addition, the developed method was applied for selective identification of bovine hemoglobin in the real samples with the recoveries of 99.0–104.7%. This proposed protocol provides a feasible and reliable strategy for preparation of the smart imprinted material with multiple stimulation responses.

中文翻译：

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一种用于选择性检测牛血红蛋白的磁性热敏荧光印迹聚合物

摘要 本文以磁性碳点为荧光源，聚（正异丙基酰胺）和N，N-亚甲基双丙烯酰胺为功能单体，制备了一种新型磁性热敏荧光印迹聚合物，用于牛血红蛋白的高选择性鉴定。和交联剂，分别。采用傅里叶变换红外光谱仪、荧光光谱仪、扫描电子显微镜、透射电子显微镜和振动样品磁强计对磁性热敏荧光印迹聚合物进行了详细表征。磁性热敏荧光印迹聚合物的荧光在牛血红蛋白浓度范围为 0.1-9.0 μM 时线性猝灭，检测限为 48.0 nM。印记因子为 2.3，这表明磁性热敏荧光印迹聚合物对牛血红蛋白具有优异的特异性。利用热敏性的优势，可以显着简化和提高牛血红蛋白的洗脱效率。此外，该方法用于选择性鉴定实际样品中的牛血红蛋白，回收率为 99.0-104.7%。该提议的协议为制备具有多重刺激响应的智能印记材料提供了一种可行且可靠的策略。将所开发的方法应用于实际样品中牛血红蛋白的选择性鉴定，回收率为99.0-104.7%。该提议的协议为制备具有多重刺激响应的智能印记材料提供了一种可行且可靠的策略。将所开发的方法应用于实际样品中牛血红蛋白的选择性鉴定，回收率为99.0-104.7%。该提议的协议为制备具有多重刺激响应的智能印记材料提供了一种可行且可靠的策略。