In this study, Fe₃O₄/nanodiamond nanocomposites (MND) were synthesized by polyglycerol-mediated covalent bonding. The horseradish peroxidase (HRP) was successfully immobilized on PG layer of MND by interaction between functional groups of MND and HRP, where the HRP molecules became tridimensionally connected outside the MND. The physicochemical properties of MND were analyzed by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy measurements, vibrating sample magnetometer, Fourier transform infrared spectroscopy and zeta potential. The optimal conditions for the immobilization of HRP by MND are 7 mg/L, 35 °C and 3 h, respectively. Then, the effects of temperature, pH and storage time on the activity of immobilized enzyme and free enzyme were studied. The results showed that the relative activities of immobilized enzymes were higher than free enzymes at different temperatures, pH and storage time. In addition, the reusability experiments of immobilized enzymes showed that after six cycles, the immobilized HRP retained a relative activity of 75%. Applied to the removal of phenol, the effects of phenol concentration, H₂O₂/phenol molar concentration, immobilized HRP concentration and temperature on phenol removal were investigated. The results displayed that the removal efficiency of phenol reached a maximum when the phenol concentration was 75 mg/L, the ratio of H₂O₂ to phenol was 1, the immobilized HRP concentration was 0.25 mg/L and the temperature was 30 °C. The above results indicate that the immobilized HRP exhibits high removal efficiency and has great potential for removing phenol.

在这项研究中，Fe ₃ O ₄纳米金刚石纳米复合材料（MND）是通过聚甘油介导的共价键合成的。辣根过氧化物酶（HRP）通过MND和HRP官能团之间的相互作用成功地固定在MND的PG层上，其中HRP分子在MND外部三维连接。通过X射线衍射，透射电子显微镜，X射线光电子能谱测量，振动样品磁力计，傅立叶变换红外光谱和Zeta电位分析了MND的理化性质。MND固定HRP的最佳条件分别为7 mg / L，35°C和3 h。然后，研究了温度，pH和储存时间对固定化酶和游离酶活性的影响。结果表明，在不同的温度，pH值和保存时间下，固定化酶的相对活性均高于游离酶。此外，固定化酶的重用性实验表明，经过六个循环后，固定化HRP保留了75％的相对活性。适用于去除苯酚，影响苯酚浓度，H_研究了2 O ₂ /苯酚摩尔浓度，固定化HRP浓度和温度对苯酚去除的影响。结果表明，当苯酚浓度为75 mg / L，H ₂ O ₂与苯酚的比例为1，固定化HRP浓度为0.25 mg / L，温度为30°C时，苯酚的去除效率达到最大值。。以上结果表明，固定化的HRP具有较高的去除效率，并且具有很大的去除苯酚的潜力。