当前位置:
X-MOL 学术
›
Microchim. Acta
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Colorimetric determination of ascorbic acid using a polyallylamine-stabilized IrO2/graphene oxide nanozyme as a peroxidase mimic
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-01-08 , DOI: 10.1007/s00604-019-3897-4 Huiyuan Sun 1, 2 , Xueliang Liu 1 , Xinhuan Wang 1 , Qiusen Han 1, 3 , Cui Qi 1 , Yanmei Li 2 , Chen Wang 1, 3 , Yongxiang Chen 2 , Rong Yang 1, 3
Microchimica Acta ( IF 5.7 ) Pub Date : 2020-01-08 , DOI: 10.1007/s00604-019-3897-4 Huiyuan Sun 1, 2 , Xueliang Liu 1 , Xinhuan Wang 1 , Qiusen Han 1, 3 , Cui Qi 1 , Yanmei Li 2 , Chen Wang 1, 3 , Yongxiang Chen 2 , Rong Yang 1, 3
Affiliation
The authors describe a peroxidase-mimicking nanozyme composed of IrO2 and graphene oxide (GO). It was synthesized from monodisperse IrO2 nanoparticles with an average diameter of 1.7 ± 0.3 nm that were prepared by pulsed laser ablation in ethanol. The nanoparticles were then placed on polyallylamine-modified GO nanosheets through electrostatic interaction. The peroxidase-like activity of the resulting nanocomposites was evaluated by catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine in the presence of H2O2. Kinetic results demonstrated that the catalytic behavior of the nanocomposites follows Michaelis-Menten kinetics. Experiments performed with terephthalic acid and cytochrome C confirmed that the peroxidase-like activity originated from the electron transfer mechanism rather than from generation of hydroxy radicals. The peroxidase-like activity is inhibited in the presence of ascorbic acid (AA). Based on this property, a colorimetric assay was developed for the determination of AA by exploiting the peroxidase-like activity of IrO2/GO nanocomposites. The linear relationship between absorbance at 652 nm and the concentration of AA was acquired. The limit of detection for AA is 324 nM. Further applications of the method for AA detection in real samples were also successfully demonstrated. Graphical abstract Schematic of the preparation of polyallylamine (PAH)-stabilized IrO2/GO nanocomposites and the colorimetric detection of AA based on the peroxidase-like activity of IrO2/GO nanocomposites. Schematic of the preparation of polyallylamine (PAH)-stabilized IrO2/GO nanocomposites and the colorimetric detection of AA based on the peroxidase-like activity of IrO2/GO nanocomposites.
中文翻译:
使用聚烯丙胺稳定的 IrO2/氧化石墨烯纳米酶作为过氧化物酶模拟物比色测定抗坏血酸
作者描述了一种由 IrO2 和氧化石墨烯 (GO) 组成的模拟过氧化物酶的纳米酶。它由平均直径为 1.7 ± 0.3 nm 的单分散 IrO2 纳米粒子合成,该纳米粒子通过脉冲激光烧蚀在乙醇中制备。然后通过静电相互作用将纳米颗粒置于聚烯丙胺改性的 GO 纳米片上。通过在 H2O2 存在下催化氧化 3,3',5,5'-四甲基联苯胺来评估所得纳米复合材料的过氧化物酶样活性。动力学结果表明纳米复合材料的催化行为遵循 Michaelis-Menten 动力学。用对苯二甲酸和细胞色素 C 进行的实验证实,过氧化物酶样活性源于电子转移机制而不是羟基自由基的产生。在抗坏血酸 (AA) 存在下,过氧化物酶样活性受到抑制。基于这一特性,开发了一种通过利用 IrO2/GO 纳米复合材料的过氧化物酶样活性来测定 AA 的比色法。获得了 652 nm 处的吸光度与 AA 浓度之间的线性关系。AA 的检测限为 324 nM。AA 检测方法在实际样品中的进一步应用也得到了成功证明。图形摘要 聚烯丙胺 (PAH) 稳定的 IrO2/GO 纳米复合材料的制备示意图和基于 IrO2/GO 纳米复合材料的过氧化物酶样活性的 AA 比色检测。
更新日期:2020-01-08
中文翻译:
使用聚烯丙胺稳定的 IrO2/氧化石墨烯纳米酶作为过氧化物酶模拟物比色测定抗坏血酸
作者描述了一种由 IrO2 和氧化石墨烯 (GO) 组成的模拟过氧化物酶的纳米酶。它由平均直径为 1.7 ± 0.3 nm 的单分散 IrO2 纳米粒子合成,该纳米粒子通过脉冲激光烧蚀在乙醇中制备。然后通过静电相互作用将纳米颗粒置于聚烯丙胺改性的 GO 纳米片上。通过在 H2O2 存在下催化氧化 3,3',5,5'-四甲基联苯胺来评估所得纳米复合材料的过氧化物酶样活性。动力学结果表明纳米复合材料的催化行为遵循 Michaelis-Menten 动力学。用对苯二甲酸和细胞色素 C 进行的实验证实,过氧化物酶样活性源于电子转移机制而不是羟基自由基的产生。在抗坏血酸 (AA) 存在下,过氧化物酶样活性受到抑制。基于这一特性,开发了一种通过利用 IrO2/GO 纳米复合材料的过氧化物酶样活性来测定 AA 的比色法。获得了 652 nm 处的吸光度与 AA 浓度之间的线性关系。AA 的检测限为 324 nM。AA 检测方法在实际样品中的进一步应用也得到了成功证明。图形摘要 聚烯丙胺 (PAH) 稳定的 IrO2/GO 纳米复合材料的制备示意图和基于 IrO2/GO 纳米复合材料的过氧化物酶样活性的 AA 比色检测。