To increase the sensitivity of electrochemical sensor, Fe-MIL-88B-NH 2 (Fe-MOF) with peroxidase-like activity is designed for the construction of immunoprobe. The Fe-MOF was prepared by one-step hydrothermalf method using 2-aminoterephthalic acid and iron(III) chloride. For the immunoprobe, it was fabricated by gold nanocomposite/Fe-MOF (Au/Fe-MOF) for the immobilization of labeling antibody (the antibody was used to conjuncting with label materials). The thin layer of Methylene Blue (MB) covered by reduced graphene oxide-gold nanocomposites (Au-rGO) serves as a substrate to covalently fix coating antibodies. The MB as a redox-active species was modified on the glass carbon electrode that can give a strong amperometric signal at 0.18 V (vs. Ag/AgCl). With the participation of H 2 O 2 , Fe-MOF can induce the Fenton reaction which degrades MB covered by Au-rGO on the substrate. The rest of MB on the surface of electrode becomes oxidized thereby generating a current signal. Square wave voltammetry (SWV) was used to quantify PSA. Under optimal conditions, the immunoassay is stable, specific and reproducible. It has a lower detection limit of 0.13 pg mL −1 (S/ N = 3) and a wide analytical range that extends from 0.001 to 100 ng mL −1 . Graphical abstract A sandwich-type amperometric immunoassay based on Fe-MOF-induced Fenton reaction was designed for sensitive determination of prostate specific antigen.

中文翻译：

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通过利用具有过氧化物酶模拟活性的 Au/Fe-MOF 型金属有机骨架纳米复合材料诱导的芬顿反应，针对前列腺特异性抗原的超灵敏电流免疫传感器

为了提高电化学传感器的灵敏度，设计了具有类过氧化物酶活性的 Fe-MIL-88B-NH 2 (Fe-MOF) 用于构建免疫探针。Fe-MOF 是通过一步水热法使用 2-氨基对苯二甲酸和氯化铁 (III) 制备的。对于免疫探针，它由金纳米复合物/Fe-MOF（Au/Fe-MOF）制成，用于固定标记抗体（抗体用于与标记材料结合）。由还原氧化石墨烯-金纳米复合材料 (Au-rGO) 覆盖的亚甲蓝 (MB) 薄层用作共价固定涂层抗体的基底。MB 作为氧化还原活性物质在玻璃碳电极上进行了修饰，可以在 0.18 V（相对于 Ag/AgCl）下提供强电流信号。在 H 2 O 2 的参与下，Fe-MOF 可以诱导 Fenton 反应，从而降解被 Au-rGO 覆盖在基材上的 MB。电极表面上的其余 MB 被氧化，从而产生电流信号。方波伏安法 (SWV) 用于量化 PSA。在最佳条件下，免疫测定是稳定、特异性和可重复的。它具有 0.13 pg mL -1 (S/N = 3) 的检测下限和从 0.001 到 100 ng mL -1 的宽分析范围。图形摘要 基于 Fe-MOF 诱导的 Fenton 反应的夹心型电流免疫测定被设计用于敏感测定前列腺特异性抗原。具体且可重复。它具有 0.13 pg mL -1 (S/N = 3) 的检测下限和从 0.001 到 100 ng mL -1 的宽分析范围。图形摘要 基于 Fe-MOF 诱导的 Fenton 反应的夹心型电流免疫测定被设计用于敏感测定前列腺特异性抗原。具体且可重复。它具有 0.13 pg mL -1 (S/N = 3) 的检测下限和从 0.001 到 100 ng mL -1 的宽分析范围。图形摘要 基于 Fe-MOF 诱导的 Fenton 反应的夹心型电流免疫测定被设计用于敏感测定前列腺特异性抗原。