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Expanded mesoporous silica-encapsulated ultrasmall Pt nanoclusters as artificial enzymes for tracking hydrogen peroxide secretion from live cells
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.aca.2020.01.015
Meng Li 1 , Jie Lv 1 , Shuangling Wang 1 , Jing Wang 1 , Yulong Lin 1
Affiliation  

Design of synthetic structures that possess the similar functions to natural enzymes held great promise in environmental detection and biomedical application. Herein, a new concept for the fabrication of solid-supported catalysts as peroxidase mimic have been proposed to realize high-catalytic activity and stability by utilizing expanded mesoporous silica (EMSN)-encapsulated Pt nanoclusters. Compared with PtNCs, the introduction of amino group modified EMSN would enrich H2O2 on the surface of PtNCs and increase the catalytic sites for H2O2 decomposition, which gave rise to the higher catalytic activity of EMSN-PtNCs over a broad pH range, especially in weakly acidic and neural solutions. This would facilitate their applications for real-time monitoring the secretion of H2O2 from living cancer cells stimulated by various anticancer drugs. Our findings not only pave the way to use porous matrix as the structural component for the design of the biomimetic catalysts, but also provide a simple and reliable platform to monitor H2O2 released from living cells in real time, which holds great potential for elucidating the biological roles of H2O2 and underlying molecular mechanisms of drug cytotoxicity as well as drug therapeutic effects.

中文翻译:

扩展的介孔二氧化硅包裹的超小 Pt 纳米团簇作为人工酶,用于追踪活细胞分泌过氧化氢

设计具有与天然酶相似功能的合成结构在环境检测和生物医学应用中具有广阔的前景。在此,提出了一种制备作为过氧化物酶模拟物的固体负载催化剂的新概念,通过利用膨胀介孔二氧化硅 (EMSN) 封装的 Pt 纳米团簇来实现高催化活性和稳定性。与 PtNCs 相比,引入氨基修饰的 EMSN 会在 PtNCs 表面富集 H2O2,增加 H2O2 分解的催化位点,这使得 EMSN-PtNCs 在较宽的 pH 范围内具有更高的催化活性,尤其是在弱酸性条件下和神经解决方案。这将有助于它们在实时监测受各种抗癌药物刺激的活癌细胞中分泌 H2O2 的应用。
更新日期:2020-04-01
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