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Whole-cell biocatalysis using cytochrome P450 monooxygenases for biotransformation of sustainable bioresources (fatty acids, fatty alkanes, and aromatic amino acids).
Biotechnology Advances ( IF 16.0 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.biotechadv.2020.107504
HyunA Park 1 , Gyuyeon Park 2 , Wooyoung Jeon 2 , Jung-Oh Ahn 3 , Yung-Hun Yang 4 , Kwon-Young Choi 1
Affiliation  

Cytochrome P450s (CYPs) are heme-thiolated enzymes that catalyze the oxidation of Csingle bondH bonds in a regio and stereoselective manner. Activation of the non-activated carbon atom can be further enhanced by multistep chemo-enzymatic reactions; moreover, several useful chemicals can be synthesized to provide alternative organic synthesis routes. Given their versatile functionality, CYPs show promise in a number of biotechnological fields. Recently, various CYPs, along with their sequences and functionalities, have been identified owing to rapid developments in sequencing technology and molecular biotechnology. In addition to these discoveries, attempts have been made to utilize CYPs to industrially produce biochemicals from available and sustainable bioresources such as oil, amino acids, carbohydrates, and lignin. Here, these accomplishments, particularly those involving the use of CYP enzymes as whole-cell biocatalysts for bioresource biotransformation, will be reviewed. Further, recently developed biotransformation pathways that result in gram-scale yields of fatty acids and fatty alkanes as well as aromatic amino acids, which depend on the hosts used for CYP expression, and the nature of the multistep reactions will be discussed. These pathways are similar regardless of whether the hosts are CYP-producing or non-CYP-producing; the limitations of these methods and the ways to overcome them are reviewed here.



中文翻译:

使用细胞色素P450单加氧酶进行全细胞生物催化,以实现可持续生物资源(脂肪酸,脂肪烷烃和芳香族氨基酸)的生物转化。

细胞色素P450(CYP)是血红素硫醇化酶,可催化C的氧化单键H以区域和立体选择性的方式键合。未活化的碳原子的活化可以通过多步化学酶促反应来进一步增强。此外,可以合成几种有用的化学物质以提供替代的有机合成路线。CYP具有通用功能,因此在许多生物技术领域都显示出了希望。近来,由于测序技术和分子生物技术的快速发展,已鉴定出各种CYP及其序列和功能。除这些发现外,还尝试利用CYP从可用的和可持续的生物资源(如油,氨基酸,碳水化合物和木质素)中工业生产生物化学药品。在这里,这些成就,特别是那些涉及使用CYP酶作为生物资源生物转化全细胞生物催化剂的研究。此外,最近开发的生物转化途径会导致脂肪酸和脂肪烷烃以及芳香族氨基酸的克级产量,这取决于用于CYP表达的宿主,并将讨论多步反应的性质。无论宿主是CYP产生者还是非CYP产生者,这些途径都是相似的。本文介绍了这些方法的局限性以及克服这些方法的方法。并将讨论多步反应的性质。无论宿主是CYP产生者还是非CYP产生者,这些途径都是相似的。本文介绍了这些方法的局限性以及克服这些方法的方法。并将讨论多步反应的性质。无论宿主是CYP产生者还是非CYP产生者,这些途径都是相似的。本文介绍了这些方法的局限性以及克服这些方法的方法。

更新日期:2020-01-08
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