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Chronic constriction injury of the sciatic nerve in rats causes different activation modes of microglia between the anterior and posterior horns of the spinal cord.
Neurochemistry international ( IF 4.2 ) Pub Date : 2020-01-09 , DOI: 10.1016/j.neuint.2020.104672
Tasuku Nishihara 1 , Junya Tanaka 2 , Keisuke Sekiya 1 , Yuki Nishikawa 3 , Naoki Abe 1 , Taisuke Hamada 1 , Sakiko Kitamura 1 , Keizo Ikemune 1 , Shinichiro Ochi 4 , Mohammed E Choudhury 2 , Hajime Yano 2 , Toshihiro Yorozuya 1
Affiliation  

Chronic constriction injury of the sciatic nerve is frequently considered as a cause of chronic neuropathic pain. Marked activation of microglia in the posterior horn (PH) has been well established with regard to this pain. However, microglial activation in the anterior horn (AH) is also strongly induced in this process. Therefore, in this study, we compared the differential activation modes of microglia in the AH and PH of the lumbar cord 7 days after chronic constriction injury of the left sciatic nerve in Wistar rats. Microglia in both the ipsilateral AH and PH demonstrated increased immunoreactivity of the microglial markers Iba1 and CD11b. Moreover, abundant CD68+ phagosomes were observed in the cytoplasm. Microglia in the AH displayed elongated somata with tightly surrounding motoneurons, whereas cells in the PH displayed a rather ameboid morphology and were attached to myelin sheaths rather than to neurons. Microglia in the AH strongly expressed NG2 chondroitin sulfate proteoglycan. Despite the tight attachment to neurons in the AH, a reduction in synaptic proteins was not evident, suggesting engagement of the activated microglia in synaptic stripping. Myelin basic protein immunoreactivity was observed in the phagosomes of activated microglia in the PH, suggesting the phagocytic removal of myelin. CCI caused both motor deficit and hyperalgesia that were evaluated by applying BBB locomotor rating scale and von Frey test, respectively. Motor defict was the most evident at postoperative day1, and that became less significant thereafter. By contrast, hyperalgesia was not severe at day 1 but it became worse at least by day 7. Collectively, the activation modes of microglia were different between the AH and PH, which may be associated with the difference in the course of motor and sensory symptoms.

中文翻译:

大鼠坐骨神经的慢性压迫性损伤导致脊髓的前角和后角之间的小胶质细胞激活方式不同。

坐骨神经的慢性压迫性损伤通常被认为是慢性神经性疼痛的原因。关于这种疼痛,后角(PH)中的小胶质细胞已明显激活。但是,在此过程中还强烈诱导了前角(AH)中的小胶质细胞活化。因此,在这项研究中,我们比较了Wistar大鼠左坐骨神经慢性压迫损伤后7天,小胶质细胞在AH和PH腰椎小胶质细胞的不同激活方式。同侧AH和PH中的小胶质细胞显示出小胶质标记Iba1和CD11b的免疫反应性增加。此外,在细胞质中观察到大量的CD68 +吞噬体。AH中的小胶质细胞显示出伸长的躯体,周围的运动神经元紧绷,而PH中的细胞表现出类似类阿贝状的形态,并附着在髓鞘上而不是神经元上。AH中的小胶质细胞强烈表达NG2硫酸软骨素蛋白聚糖。尽管AH中神经元紧密附着,但突触蛋白的减少并不明显,这表明活化的小胶质细胞参与突触剥离。在PH中,在活化的小胶质细胞的吞噬体中观察到髓磷脂碱性蛋白的免疫反应性,表明吞噬作用去除了髓磷脂。CCI引起运动功能障碍和痛觉过敏,分别通过应用BBB运动评分量表和von Frey检验进行评估。术后1天运动偏转最明显,此后此变化变得不那么明显。相比之下,痛觉过敏在第1天并不严重,但至少在第7天时加剧。
更新日期:2020-01-09
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