Osteoarthritis (OA) is one of the most common diseases worldwide caused by chronic degeneration of the joints. Its high prevalence and the involvement of several tissues define OA as a highly heterogeneous disease. New biological markers to evaluate the progression of the pathology and improve its prognosis are needed. Among all the different -omic strategies applied to OA, solution phase bottom-up proteomics has made an extensive contribution to the field of biomarker research. However, new technologies for protein analyses should be considered for a better understanding of the disease. This review focuses on complementary proteomic methodologies and new technologies for translational research of OA and other rheumatic pathologies, especially mass spectrometry imaging and protein imaging methods not applied by the OA community yet.

骨关节炎（OA）是全世界由关节的慢性变性引起的最常见疾病之一。它的高患病率和多个组织的参与将OA定义为高度异质性疾病。需要新的生物学标记物来评估病理学进展并改善其预后。在应用于OA的所有不同组学策略中，溶液相自下而上的蛋白质组学已为生物标志物研究领域做出了巨大贡献。但是，应考虑使用蛋白质分析的新技术以更好地了解该疾病。这篇综述着重于OA和其他风湿性疾病的转化研究的补充蛋白质组学方法和新技术，特别是OA社区尚未应用的质谱成像和蛋白质成像方法。