The influence of electrostatic conditions (salt concentration of the solution and vesicle surface charge density) on the size distribution of self-assembled giant unilamellar vesicles (GUVs) is considered. The membranes of GUVs are synthesized by a mixture of dioleoylphosphatidylglycerol and dioleoylphosphatidylcholine in a physiological buffer using the natural swelling method. The experimental results are presented in the form of a set of histograms. The log-normal distribution is used for statistical treatment of results. It is obtained that the decrease of salt concentration and the increase of vesicle surface charge density of the membranes increase the average size of the GUV population. To explain the experimental results, a theory using the Helmholtz free energy of the system describing the GUV vesiculation is developed. The size distribution histograms and average size of GUVs under various conditions are fitted with the proposed theory. It is shown that the variation of the bending modulus due to changing of electrostatic parameters of the system is the main factor causing a change in the average size of GUVs.

中文翻译：

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静电相互作用对自组装巨型单层囊泡大小分布的影响。

考虑了静电条件（溶液的盐浓度和囊泡表面电荷密度）对自组装巨型单层囊泡（GUV）的尺寸分布的影响。使用自然溶胀法，通过在生理缓冲液中的油酰磷脂酰甘油甘油和油酰磷脂酰胆碱的混合物合成GUV的膜。实验结果以一组直方图的形式呈现。对数正态分布用于结果的统计处理。可以看出，盐浓度的降低和膜囊泡表面电荷密度的增加会增加GUV群体的平均大小。为了解释实验结果，建立了使用亥姆霍兹自由能的系统描述GUV囊泡形成的理论。在各种条件下，GUV的尺寸分布直方图和平均尺寸都符合所提出的理论。结果表明，由于系统静电参数的变化，弯曲模量的变化是引起GUV平均尺寸变化的主要因素。