Vibrio harveyi is the pathogen causing vibriosis in marine-cultured animals, leading to massive deaths in farmed grouper around the world. It is urgent to develop an effective vaccine to prevent vibriosis. In the previous study, we developed a V. harveyi formalin-killed cells vaccine (FKC), and sought an effective adjuvant for enhancing the immune efficacy of vaccine. In this study, we aimed to evaluate the immune responses and protective effect of FKC combined with chitosan oligosaccharide (COS) or Astragalus polysaccharides (APS) in the pearl gentian grouper♀Epinephelus fuscoguttatus × ♂E. lanceolatus. The results indicated the vaccine triggered a remarkably higher expression levels of IL-1β, IL-16, TNF-α, MHC-Iα and IgM in the kidney and spleen of groupers post-vaccination. Antibody titers, lysozyme, catalase, superoxide dismutase and total protein were significantly elevated in the vaccinated fish compared with those in the control. The experimental groupers were challenged intraperitoneally by V. harveyi at 35 d post-vaccination, and the relative percentage of survival (RPS) of group FKC + COS, FKC + APS, COS, APS and FKC were 80%, 72%, 52%, 47% and 55%, respectively. These results demonstrated COS and APS was the potential adjuvants for FKC against V. harveyi in aquaculture.

中文翻译：

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哈氏弧菌福尔马林灭活细胞疫苗联合壳聚糖低聚糖和黄芪多糖对♀草石脑鱼×lance草石脑鱼的免疫作用。

哈维氏弧菌是导致海洋养殖动物发生弧菌病的病原体，导致全世界养殖的石斑鱼大量死亡。迫切需要开发一种有效的疫苗来预防弧菌病。在先前的研究中，我们开发了哈氏弧菌福尔马林杀死的细胞疫苗（FKC），并寻求有效的佐剂来增强疫苗的免疫功效。在这项研究中，我们旨在评估FKC与壳聚糖寡糖（COS）或黄芪多糖（APS）联合对珍珠龙胆石斑鱼pine褐飞虱（Epinephelus fuscoguttatus×♂E）的免疫应答和保护作用。lanceolatus。结果表明，疫苗在接种后的石斑鱼的肾脏和脾脏中触发了IL-1β，IL-16，TNF-α，MHC-Iα和IgM的高表达水平。抗体滴度，溶菌酶，过氧化氢酶，与对照组相比，接种疫苗的鱼中超氧化物歧化酶和总蛋白显着升高。接种疫苗后35 d，哈维氏菌腹膜内攻击实验石斑鱼，FKC + COS，FKC + APS，COS，APS和FKC组的相对存活率（RPS）分别为80％，72％，52％ ，分别为47％和55％。这些结果表明，COS和APS是FKC对抗水产养殖哈氏弧菌的潜在佐剂。