Induction of beige adipocytes in white adipose tissue (WAT) is a potential therapeutic target for the treatment of obesity because beige adipocytes release excess energy via uncoupling-protein-1-associated thermogenesis. In this study, we investigated how artepillin C (ArtC) promotes thermogenesis in vivo. We demonstrated that 28 day administration of ArtC (10 mg/kg of body weight) to mice significantly induced thermogenesis in beige adipocytes in inguinal WAT (iWAT) and suppressed reductions in core body temperature induced by cold exposure at 4 °C. Moreover, ArtC-induced thermogenesis in iWAT was significantly inhibited by treatment with a creatine metabolism inhibitor, and ArtC significantly upregulated the expression of creatine-metabolism-related enzymes in the thermogenic pathway. These results indicate that ArtC induces thermogenesis in beige adipocytes in iWAT, and the observed ArtC-induced thermogenesis is associated with the creatine-metabolism-related thermogenic pathway, which is characteristically observed in beige adipocytes.

中文翻译：

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巴西蜂胶的关键成分Artepillin C通过与肌酸代谢相关的生热途径诱导小鼠腹股沟白色脂肪组织的生热作用。

在白色脂肪组织（WAT）中诱导米色脂肪细胞是治疗肥胖症的潜在治疗靶标，因为米色脂肪细胞通过与蛋白质1相关的解热作用释放多余的能量。在这项研究中，我们调查了青霉素C（ArtC）如何促进体内生热。我们证明，对小鼠进行28天ArtC（10 mg / kg体重）给药可显着诱导腹股沟WAT（iWAT）中米色脂肪细胞的生热作用，并抑制4°C的冷暴露诱导的核心体温降低。此外，用肌酸代谢抑制剂治疗可显着抑制ArtC诱导的iWAT中的生热作用，ArtC可显着上调热源途径中肌酸代谢相关酶的表达。