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Stimuli-Responsive Fluorescent Hyperbranched Poly(amido amine)s for Biosensing Applications
European Polymer Journal ( IF 6 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.eurpolymj.2020.109486
Tanveer-Ul-Hassan Shah , Mudassir Hussain Tahir , Muhammad Sajjad Ahmad , Ata ur Rahman , Muhammad Arshad kamran , Hewen Liu

Abstract Hyperbranched Poly(amido amine)s (PAMAM) especially those containing disulfide groups have been highly investigated for DNA condensation, drugs and genes delivery. However, the detailed optical properties of PAMAM, intrinsic fluorescence characteristics and their applications for detection of thiols in pure aqueous medium have not been explored yet. Here we report for the first time the detailed investigation of optical properties of PAMAM and their biosensing application in pure aqueous medium. Three PAMAM named as MBAP (without disulfide group), CBAP and HPAP (with disulfide) groups were chosen, synthesized and characterized. Quantum yields of these PAMAM at different pH value were calculated in pure aqueous medium and all the PAMAM were found to be pH sensitive due to presence of tertiary amine and have shown increase in quantum yields in acidic medium. MBAP was not found to be redox sensitive due to the absence of disulfide group while CBAP has shown low redox sensitivity in aqueous medium because of low water solubility. HPAP has shown high redox sensitivity and high quantum yields hence applied for detection of thiols with the limit of detection of 5.29 mM in pure aqueous medium under physiological conditions. These investigations have not only shown the detailed optical properties of extremely important PAMAM but also broadens their application for bioimaging and biosensing.

中文翻译:

用于生物传感应用的刺激响应荧光超支化聚(酰胺胺)

摘要 超支化聚(酰胺胺)(PAMAM),尤其是那些含有二硫键的物质,已被广泛研究用于 DNA 缩合、药物和基因传递。然而,尚未探索 PAMAM 的详细光学特性、固有荧光特性及其在纯水介质中检测硫醇的应用。在这里,我们首次详细研究了 PAMAM 的光学特性及其在纯水介质中的生物传感应用。选择、合成并表征了三种PAMAM,分别命名为MBAP(无二硫键)、CBAP和HPAP(有二硫键)。在纯水性介质中计算了这些 PAMAM 在不同 pH 值下的量子产率,发现所有 PAMAM 由于叔胺的存在而对 pH 敏感,并且在酸性介质中显示出量子产率的增加。由于不存在二硫化物基团,未发现 MBAP 对氧化还原敏感,而 CBAP 由于水溶性低而在水性介质中显示出低氧化还原敏感性。HPAP 已显示出高氧化还原灵敏度和高量子产率,因此应用于检测硫醇,生理条件下纯水介质中的检测限为 5.29 mM。这些研究不仅展示了极其重要的 PAMAM 的详细光学特性,而且还拓宽了它们在生物成像和生物传感方面的应用。由于不存在二硫化物基团,未发现 MBAP 对氧化还原敏感,而 CBAP 由于水溶性低而在水性介质中显示出低氧化还原敏感性。HPAP 已显示出高氧化还原灵敏度和高量子产率,因此应用于检测硫醇,生理条件下纯水介质中的检测限为 5.29 mM。这些研究不仅展示了极其重要的 PAMAM 的详细光学特性,而且还拓宽了它们在生物成像和生物传感方面的应用。由于不存在二硫化物基团,未发现 MBAP 对氧化还原敏感,而 CBAP 由于水溶性低而在水性介质中显示出低氧化还原敏感性。HPAP 已显示出高氧化还原灵敏度和高量子产率,因此应用于检测硫醇,生理条件下纯水介质中的检测限为 5.29 mM。这些研究不仅展示了极其重要的 PAMAM 的详细光学特性,而且还拓宽了它们在生物成像和生物传感方面的应用。
更新日期:2020-02-01
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