Photodynamic therapy is an emerging noninvasive cancer treatment approach, which requires a photosensitizer (PS), light, and molecular oxygen. In this study, we have successfully fabricated a dual nature (pH- and reactive-oxygen-species-responsive) upconversion nanoparticles (UCNPs) to utilize coloaded doxorubicin (DOX) and chlorin e6 (Ce6) with high antitumor efficacy. The model anticancer drug (DOX) and PS (Ce6) were conjugated in a ratio of 1:1 (w:w), and then loaded on the surface of [email protected] silica (mSiO₂) (85.63 ± 9.87 nm). Cellular uptake could be achieved by either increased permeability or ionic effect of [email protected]₂, where Ce6 controlled the DOX release under a near-infrared (NIR) laser irradiation at 980 nm. A cytotoxicity analysis revealed that the dual-responsive [email protected]₂ could successfully deliver DOX and Ce6 at the tumor site, causing cell death with a high efficiency. This study shows that the modified [email protected]₂ is a promising system to realize NIR-light-triggered PS and drug delivery approach to improve synergistic therapies in vitro and in vivo, in the future.

光动力疗法是一种新兴的非侵入性癌症治疗方法，需要光敏剂（PS），光和分子氧。在这项研究中，我们成功地制造了具有双重性质（pH和反应性氧物种响应）的上转换纳米颗粒（UCNP），以利用具有高抗肿瘤功效的共载阿霉素（DOX）和二氢卟酚e6（Ce6）。将模型抗癌药（DOX）和PS（Ce6）以1：1（w：w）的比例结合，然后加载到[电子邮件保护的]二氧化硅（mSiO ₂）（85.63±9.87 nm）的表面上。细胞的吸收可以通过提高[电子邮件保护的] _2的通透性或离子效应来实现，其中Ce6在980 nm的近红外（NIR）激光辐射下控制DOX的释放。细胞毒性分析显示，双重反应[受电子邮件保护] ₂可以成功地在肿瘤部位递送DOX和Ce6，从而高效导致细胞死亡。这项研究表明，改进的[电子邮件保护] ₂是实现NIR光触发PS和药物输送方法以改善体外和体内协同治疗的有希望的系统。