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A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia.
Comparative Biochemistry and Physiology A: Molecular & Integrative Physiology ( IF 2.3 ) Pub Date : 2020-01-08 , DOI: 10.1016/j.cbpa.2020.110649
Natalia G Li 1 , Jantina Toxopeus 2 , Martin Moos 3 , Jesper G Sørensen 4 , Brent J Sinclair 2
Affiliation  

Low temperatures limit the distribution and abundance of ectotherms. However, many insects can survive low temperatures by employing one of two cold tolerance strategies: freeze avoidance or freeze tolerance. Very few species can employ both strategies, but those that do provide a rare opportunity to study the mechanisms that differentiate freeze tolerance and freeze avoidance. We showed that overwintering pupae of the cabbage white butterfly Pieris rapae can be freeze tolerant or freeze avoidant. Pupae from a population of P. rapae in northeastern Russia (Yakutsk) froze at c. -9.3 °C and were freeze-tolerant in 2002-2003 when overwintered outside. However, P. rapae from both Yakutsk and southern Canada (London) acclimated to milder laboratory conditions in 2014 and 2017 froze at lower temperatures (< -20 °C) and were freeze-avoidant. Summer-collected P. rapae larvae (collected in Yakutsk in 2016) were partially freeze-tolerant, and decreased the temperature at which they froze in response to starvation at mild low temperatures (4 °C) and repeated partial freezing events. By comparing similarly-acclimated P. rapae pupae from both populations, we identified molecules that may facilitate low temperature tolerance, including the hemolymph ice-binding molecules and several potential low molecular weight cryoprotectants. Pieris rapae from Yakutsk exhibited high physiological plasticity, accumulating cryoprotectants and almost doubling their hemolymph osmolality when supercooled to -15 °C for two weeks, while the London P. rapae population exhibited minimal plasticity. We hypothesize that physiological plasticity is an important adaptation to extreme low temperatures (i.e. in Yakutsk) and may facilitate the transition between freeze avoidance and freeze tolerance.

中文翻译:

来自加拿大安大略省和俄罗斯远东地区雅库特的菜青虫的低温生物学比较。

低温限制了等温线的分布和数量。但是,许多昆虫可以通过采用以下两种耐寒策略之一来在低温下生存:避免冷冻或耐冷冻。极少数物种可以同时使用这两种策略,但确实有两种策略提供了难得的机会来研究区分冷冻耐受性和避免冷冻的机制。我们表明,卷心菜白蝶菜青虫的越冬p可以耐冻或避免冻害。来自俄罗斯东北部(雅库茨克)的菜青虫种群的population在c。-9.3°C,2002-2003年在室外越冬时耐冻。但是,雅库茨克和加拿大南部(伦敦)的菜青虫在2014年和2017年适应较温和的实验室条件,因此在较低的温度(<-20°C)下冻结并避免冷冻。夏季采集的菜青虫幼虫(2016年在雅库茨克采集)具有一定的耐冻性,在温和的低温(4°C)下饥饿并反复发生部分冻结事件后,它们降低了冻结的温度。通过比较两个种群的相似适应的小菜蛾,,我们鉴定了可能有助于低温耐受的分子,包括血淋巴冰结合分子和几种潜在的低分子量冷冻保护剂。来自雅库茨克的菜青虫表现出很高的生理可塑性,当过冷至-15°C两周时,它们会积聚冷冻保护剂,并且其血淋巴重量摩尔渗透压浓度几乎翻倍,而伦敦菜青虫种群的可塑性却很小。我们假设生理可塑性是对极端低温的重要适应(即
更新日期:2020-01-08
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