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Cross-Linked Gelatin-Nanocellulose Scaffolds for Bone Tissue Engineering
Materials Letters ( IF 3 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.matlet.2020.127326 Ingeborg Elisabeth Carlström , Ahmad Rashad , Elisabetta Campodoni , Monica Sandri , Kristin Syverud , Anne Isine Bolstad , Kamal Mustafa
Materials Letters ( IF 3 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.matlet.2020.127326 Ingeborg Elisabeth Carlström , Ahmad Rashad , Elisabetta Campodoni , Monica Sandri , Kristin Syverud , Anne Isine Bolstad , Kamal Mustafa
Abstract Wood-based cellulose nanofibrils (CNFs) have, in addition to high specific surface area and high surface reactivity, ability to mimic nanostructured collagen in bone extracellular matrix. These properties make CNFs promising materials for bone tissue engineering (BTE). The CNFs degrade slowly in vivo. By blending and cross-linking gelatin (Gel) with CNFs, scaffolds were produced with tuned degradation rate and enhanced mechanical properties, more suitable for BTE applications. This in vitro study aimed to examine initial biological responses of human bone marrow mesenchymal stem cells to cross-linked Gel-CNF scaffolds. The scaffolds were fabricated from 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-oxidized CNF blended with Gel and cross-linked either by dehydrothermal treatment (DHT) or by a combination of hexamethylenediamine, genipin, and DHT. CNF scaffolds without cross-linking served as control. The produced scaffolds supported cell attachment, spreading, and osteogenic differentiation. However, the early cell attachment after 1 day and the expression of RUNX2 and SPP1 genes after 7 days were highest in the CNF scaffolds. The results suggest that cross-linked Gel-CNF are cytocompatible and holds potential for BTE applications.
中文翻译:
用于骨组织工程的交联明胶-纳米纤维素支架
摘要 木质纤维素纳米纤维 (CNF) 除了具有高比表面积和高表面反应性外,还具有模拟骨细胞外基质中纳米结构胶原蛋白的能力。这些特性使 CNF 成为骨组织工程 (BTE) 的理想材料。CNFs 在体内缓慢降解。通过将明胶 (Gel) 与 CNF 混合和交联,生产的支架具有可调节的降解速率和增强的机械性能,更适合 BTE 应用。这项体外研究旨在检查人骨髓间充质干细胞对交联 Gel-CNF 支架的初始生物学反应。支架由 2,2,6,6-四甲基哌啶-1-氧基(TEMPO)氧化的 CNF 与凝胶混合制成,并通过脱水热处理(DHT)或六亚甲基二胺的组合进行交联,京尼平和 DHT。没有交联的 CNF 支架作为对照。产生的支架支持细胞附着、扩散和成骨分化。然而,1 天后的早期细胞附着以及 7 天后 RUNX2 和 SPP1 基因的表达在 CNF 支架中最高。结果表明,交联的 Gel-CNF 具有细胞相容性,并具有 BTE 应用的潜力。
更新日期:2020-04-01
中文翻译:
用于骨组织工程的交联明胶-纳米纤维素支架
摘要 木质纤维素纳米纤维 (CNF) 除了具有高比表面积和高表面反应性外,还具有模拟骨细胞外基质中纳米结构胶原蛋白的能力。这些特性使 CNF 成为骨组织工程 (BTE) 的理想材料。CNFs 在体内缓慢降解。通过将明胶 (Gel) 与 CNF 混合和交联,生产的支架具有可调节的降解速率和增强的机械性能,更适合 BTE 应用。这项体外研究旨在检查人骨髓间充质干细胞对交联 Gel-CNF 支架的初始生物学反应。支架由 2,2,6,6-四甲基哌啶-1-氧基(TEMPO)氧化的 CNF 与凝胶混合制成,并通过脱水热处理(DHT)或六亚甲基二胺的组合进行交联,京尼平和 DHT。没有交联的 CNF 支架作为对照。产生的支架支持细胞附着、扩散和成骨分化。然而,1 天后的早期细胞附着以及 7 天后 RUNX2 和 SPP1 基因的表达在 CNF 支架中最高。结果表明,交联的 Gel-CNF 具有细胞相容性,并具有 BTE 应用的潜力。