Ceratocystis fimbriata is an important plant pathogen known to cause Ceratocystis Wilt (CW), a prevalent fungal disease known to affect Eucalyptus spp. plantations in Brazil. To better understand the molecular mechanisms related to pathogenicity in eucalyptus, we generated a high-quality assembly and annotation of the Ce. fimbriata LPF1912 isolate (LPF1912) genome, as well as the first transcriptome of LPF1912 from 16 eucalyptus clones at three infection incubation periods (12, 18, and 24 h). The LPF1912 genome assembly contains 805 scaffolds, totaling 31.8 Mb, with 43% of the genome estimated to be coding sequence comprised of 7,390 protein-coding genes of which 626 (8.5%) were classified as secreted proteins, 120 ribosomal RNAs, and 532 transfer RNAs. Comparative genomic analysis among three eucalyptus fungal pathogens (Ce. fimbriata, Ce. eucalypticola, and Calonectria pseudoreteaudii), showed high similarity in the proteome (21.81%) and secretome (52.01%) of LPF1912 and Ce. eucalypticola. GO annotation of pathogenicity-related genes of LPF1912 and Ce. eucalypticola, revealed enrichment in cell wall degrading enzymes (CWDEs), and lipid/cutin metabolism for Ca. pseudoreteaudii. Additionally, a transcriptome analysis between resistant and susceptible eucalyptus clones to CW infection indicated that a majority (11) of LPF1912 differentially expressed genes had GO terms associated with enzymatic functions, such as the polygalacturonase gene family, confirming the crucial role of CWDEs for Ce. fimbriata pathogenicity. Finally, our genomic and transcriptomic analysis approach provides a better understanding of the mechanisms involved in Ce. fimbriata pathogenesis, as well as a framework for further studies.

中文翻译：

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比较的基因组和转录组分析揭示了三种桉树真菌病原体之间不同的致病性相关基因。

纤维状cerercystcystis fimbriata是一种重要的植物病原体，已知会引起Ceratocystis Wilt（CW），Ceratocystis Wilt（CW）是一种常见的真菌病，已知会影响桉树。巴西的人工林。为了更好地了解与桉树致病性相关的分子机制，我们生成了Ce的高质量装配和注释。在三个感染潜伏期（12、18和24小时），从16个桉树克隆中分离出了纤毛虫LPF1912分离株（LPF1912）基因组以及LPF1912的第一个转录组。LPF1912基因组装配体包含805个支架，总计31.8 Mb，其中估计有43％的基因组是由7,390个蛋白编码基因组成的编码序列，其中626个（8.5％）被分类为分泌蛋白，120个核糖体RNA和532个转移RNA。三种桉树真菌病原体（Ce。fimbriata，Ce。LPF1912和Ce的蛋白质组（21.81％）和分泌组（52.01％）具有很高的相似性。桉树。LPF1912和Ce的致病性相关基因的GO注释。桉树，揭示了细胞壁降解酶（CWDEs）的富集以及Ca的脂质/角质代谢。假reteaudii。此外，在对CW感染具有抗性和敏感性的桉树克隆之间进行的转录组分析表明，大多数（11）LPF1912差异表达的基因具有与酶功能相关的GO术语，例如多半乳糖醛酸酶基因家族，证实了CWDE对Ce具有至关重要的作用。菌毛致病性。最后，我们的基因组和转录组分析方法可以更好地了解Ce涉及的机制。纤维菌病的发病机理